NATURAL, GENETICALLY-DETERMINED RESISTANCE TOWARD INFLUENZA-VIRUS IN HEMATOPOIETIC MOUSE CHIMERAS - ROLE OF MONONUCLEAR PHAGOCYTES
NATURAL, GENETICALLY-DETERMINED RESISTANCE TOWARD INFLUENZA-VIRUS IN HEMATOPOIETIC MOUSE CHIMERAS - ROLE OF MONONUCLEAR PHAGOCYTES
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DOI：
10.1084/jem.150.1.117
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发表时间：
1979-01-01
影响因子：
15.3
通讯作者：
LINDENMANN, J
中科院分区：
文献类型：
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作者：
HALLER, O;ARNHEITER, H;LINDENMANN, J
通过在组织相容性小鼠中交叉转移骨髓细胞产生的辐射嵌合体，这些小鼠对多种粘病毒的致死攻击敏感或具有遗传抗性，用于测试巨噬细胞抗性（体外评估）和Mx基因引起的动物抗性（体内测量）是否存在因果关系。分别测试了嵌合体 (49) 的巨噬细胞对 M-TUR（一种禽流感病毒 A/土耳其/英格兰/63 株，适合在培养的小鼠腹膜巨噬细胞中生长）的体外攻击的抵抗力，以及动物体内对嗜肺、嗜神经或嗜肝流感病毒攻击后的抵抗力。从嵌合小鼠中收获的培养的库普弗细胞和腹膜巨噬细胞表达骨髓供体的抗性表型，无论它们分化的宿主环境如何。体内的敏感性或耐药性取决于宿主的基因型。辐射嵌合体的先天抗性不依赖于造血系统细胞中的Mx基因表达。
Radiation chimeras produced by crosswise transfers of bone-marrow cells among histocompatible mice susceptible or genetically resistant to lethal challenge by a number of myxoviruses were used to test whether macrophage resistance (as assessed in vitro) and resistance of the animal (as measured in vivo) brought about by the gene Mx were causally related. Chimeras (49) were tested individually for resistance of their macrophages to in vitro challenge with M-TUR (a strain of avian influenza virus A/Turkey/England/63 adapted to grow in cultured mouse peritoneal macrophages) and for resistance of the animal in vivo upon challenge with pneumotropic, neurotropic or hepatotropic influenza viruses. Cultivated Kupffer cells and peritoneal macrophages harvested from chimeric mice expressed the resistance phenotype of the bone-marrow donor irrespective of the host environment in which they had differentiated. Susceptibility or resistance in vivo was according to the genotype of the host. Inborn resistance of radiation chimeras was independent of Mx-gene expression in cells of the hemopoietic system.