Structural biology. Up close with membrane lipid-protein complexes.
Structural biology. Up close with membrane lipid-protein complexes.
复制标题
结构生物学。
DOI：
10.1126/science.1214084
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Whitelegge,Julian
中科院分区：
文献类型：
--
作者：
Whitelegge,Julian
细胞及其内部的细胞器被脂质双层膜包围，脂质双层膜分隔生化反应和途径。膜嵌入的蛋白质控制分子、能量和信息的流动，使得隔离的区室充当统一的活细胞。传统上，膜蛋白被认为完全独立于周围的脂质而漂浮，然而，当描述这种流体马赛克模型时，辛格和尼科尔森认为“一小部分脂质可能与膜蛋白发生特异性相互作用”。人们花了40年的时间才充分认识到这一论断的重要性。在本期第 380 页，Zhouet al. 报告了从双层中溶解的完整完整膜蛋白复合物的质谱分析。结果表明，特定结构的脂质仍然结合在气相中并且可以进行计数。
Cells, and the organelles within them, are surrounded by lipid-bilayer membranes that compartmentalize biochemical reactions and pathways. Membrane-embedded proteins control the flux of molecules, energy, and information such that the segregated compartments function as a unified living cell. Traditionally, membrane proteins were pictured as floating around quite independently of the surrounding lipids, yet when this fluid-mosaic model was described, Singer and Nicolson qualified that “a small fraction of the lipid may interact specifically with the membrane proteins” . It has taken 40 years to fully appreciate the importance of this assertion. On page 380 of this issue, Zhouet al.report mass spectrometry of intact integral membrane protein complexes solubilized from bilayers. The results show that specific structural lipids remain bound in the gas phase and can be counted.