Preparation of highly active (Na+ + K+)-ATPase from the outer medulla of rabbit kidney.
Preparation of highly active (Na+ + K+)-ATPase from the outer medulla of rabbit kidney.
复制标题
兔肾外髓质高活性（Na K ）-ATP酶的制备。
DOI：
10.1016/0006-291x(69)90877-8
复制
发表时间：
1969
影响因子：
3.1
通讯作者：
Jens Christian Skou
中科院分区：
文献类型：
--
作者：
P. L. Jørgensen;Jens Christian Skou
本文描述了如何从兔肾外髓质纯化(Na++ K+)- atp酶，在37℃下，其比活性为881±25 mol Pi/mg蛋白/小时。该过程包括脱氧胆酸处理微粒体片段，随后通过差速离心和蔗糖梯度离心进行分离。该制剂的“纯度”估计为31-61%。
It is described how (Na++ K+)-ATPase from the outer medulla of rabbit kidney was purified to a specific activity of 881 ± 25 moles Pi/mg protein per hr at 37 C. The procedure consists of treatment of a microsomal fraction with deoxycholate and subsequent fractionation by differential centrifugation and sucrose gradient centrifugation. The “purity” of the preparation was estimated to be 31–61%.