Quake: quality-aware detection and correction of sequencing errors.
Quake: quality-aware detection and correction of sequencing errors.
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DOI:
10.1186/gb-2010-11-11-r116
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发表时间:
2010
期刊:
影响因子:
12.3
通讯作者:
Salzberg SL
中科院分区:
文献类型:
--
作者:
Kelley DR;Schatz MC;Salzberg SL
We introduce Quake, a program to detect and correct errors in DNA sequencing reads. Using a maximum likelihood approach incorporating quality values and nucleotide specific miscall rates, Quake achieves the highest accuracy on realistically simulated reads. We further demonstrate substantial improvements in de novo assembly and SNP detection after using Quake. Quake can be used for any size project, including more than one billion human reads, and is freely available as open source software from http://www.cbcb.umd.edu/software/quake.
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