Maternally Recruited DCP1A and DCP2 Contribute to Messenger RNA Degradation During Oocyte Maturation and Genome Activation in Mouse
Maternally Recruited DCP1A and DCP2 Contribute to Messenger RNA Degradation During Oocyte Maturation and Genome Activation in Mouse
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DOI：
10.1095/biolreprod.112.105312
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发表时间：
2013-01-01
影响因子：
3.6
通讯作者：
Schultz, Richard M.
中科院分区：
文献类型：
--
作者：
Ma, Jun;Flemr, Matyas;Schultz, Richard M.
卵母细胞到受精卵的转变需要将高度分化的卵母细胞转化为全能卵裂球，并且是必须成功跨越以继续发育的最早障碍之一。母体mRNA的降解可能是这种转变的核心，其特征在于卵母细胞成熟过程中从mRNA稳定性到不稳定性的转变。虽然在成熟过程中卵母细胞特异性RNA结合蛋白MSY2的磷酸化参与使母体mRNA更容易降解，但卵母细胞成熟过程中mRNA降解的机制仍然知之甚少。我们报告说，DCP 1A和DCP 2，蛋白负责decapping mRNA，编码的母体mRNA通过位于其30个非翻译区的细胞质多聚腺苷酸化元件在成熟过程中翻译招募。DCP1A和DCP2在成熟过程中都被磷酸化，CDC2A可能是负责两者的激酶，尽管MAPK可能参与DCP1A磷酸化。通过RNA干扰或吗啉代抑制DCP 1A和DCP 2的积累不仅降低了减数分裂成熟过程中mRNA的降解，而且降低了合子基因组的转录。结果表明，母亲招募的DCP 1A和DCP 2是关键球员在从mRNA的稳定性过渡到减数分裂成熟过程中的不稳定性和适当的母体mRNA降解必须成功地执行卵母细胞到合子的过渡。
The oocyte-to-zygote transition entails transforming a highly differentiated oocyte into totipotent blastomeres and represents one of the earliest obstacles that must be successfully hurdled for continued development. Degradation of maternal mRNAs, which likely lies at the heart of this transition, is characterized by a transition from mRNA stability to instability during oocyte maturation. Although phosphorylation of the oocyte-specific RNA-binding protein MSY2 during maturation is implicated in making maternal mRNAs more susceptible to degradation, mechanisms underlying mRNA degradation during oocyte maturation remain poorly understood. We report that DCP1A and DCP2, proteins responsible for decapping mRNA, are encoded by maternal mRNAs recruited for translation during maturation via cytoplasmic polyadenylation elements located in their 30 untranslated regions. Both DCP1A and DCP2 are phosphorylated during maturation, with CDC2A being the kinase likely responsible for both, although MAPK may be involved in DCP1A phosphorylation. Inhibiting accumulation of DCP1A and DCP2 by RNA interference or morpholinos decreases not only degradation of mRNAs during meiotic maturation but also transcription of the zygotic genome. The results indicate that maternally recruited DCP1A and DCP2 are critical players in the transition from mRNA stability to instability during meiotic maturation and that proper maternal mRNA degradation must be successful to execute the oocyte-to-zygote transition.