Assessment of the MicroRNA system in salt-sensitive hypertension
Assessment of the MicroRNA system in salt-sensitive hypertension
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DOI：
10.1291/hypres.28.819
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发表时间：
2005-10-01
影响因子：
5.4
通讯作者：
Iwai, N
中科院分区：
文献类型：
--
作者：
Naraba, H;Iwai, N
大多数动物microRNA与其mRNA靶点不完全互补，并通过未知机制抑制蛋白质合成。据报道，MicroRNA在许多生物过程中发挥重要作用。我们评估了Dahl盐敏感大鼠的microRNA系统，以研究microRNA在盐敏感性高血压中的可能作用。我们从Dahl盐敏感大鼠和刘易斯大鼠的肾脏中构建了microRNA文库，并确定了91个先前报道的和12个新的microRNA在肾脏中表达。然后，我们使用北方印迹法评估肾脏和心室中118种微小RNA的表达水平。在4组中未观察到microRNA表达谱的显著差异。因此，microRNA系统似乎不太可能导致Dahl盐敏感大鼠的盐敏感性高血压。
Most animal microRNAs are imperfectly complementary to their mRNA targets and inhibit protein synthesis through an unknown mechanism. MicroRNAs have been reported to play important roles in a number of biological processes. We assessed the microRNA system in Dahl salt-sensitive rats in order to investigate possible roles of microRNA in salt-sensitive hypertension. We constructed microRNA libraries from the kidneys of Dahl salt-sensitive and Lewis rats taking normal or high-salt diets (4 groups), and identified 91 previously reported and 12 new microRNAs expressed in the kidney. We then used Northern blotting to assess the expression levels of 118 microRNAs in the kidneys and heart ventricles. No significant differences in microRNA expression profiles were observed among the 4 groups. Thus, the microRNA system seemed to be unlikely to contribute to salt-sensitive hypertension in Dahl salt-sensitive rats.