REACTIVITY OF ISOLATED LIPOPOLYSACCHARIDES OF ENTEROBACTERIAL R-MUTANTS WITH COMPLETE OR INCOMPLETE CORE STRUCTURES WITH LECTINS
REACTIVITY OF ISOLATED LIPOPOLYSACCHARIDES OF ENTEROBACTERIAL R-MUTANTS WITH COMPLETE OR INCOMPLETE CORE STRUCTURES WITH LECTINS
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DOI：
10.1016/s0172-5599(80)80053-8
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
MAYER, H
中科院分区：
文献类型：
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作者：
AHAMED, NM;RADZIEJEWSKALEBRECHT, J;MAYER, H
通过琼脂凝胶和微毛细管沉淀研究了一系列具有完整和不完整核心结构的肠杆菌R突变体的碱处理脂多糖与3种选择的植物凝集素的反应性，伴刀豆球蛋白A、蓖麻凝集素和麦胚凝集素。具有完整R核心的6个已建立的核心类型的R脂多糖通过其特征凝集素反应模式彼此区分。一种核心类型与所有3种凝集素均无反应。具有不完整核心结构的R脂多糖显示了从其个体化学构型可预测的反应模式。半抗原（T1，T2或ECA）或由一个单一的重复单元（SR突变体）取代完整的R核心没有改变的反应模式，虽然一些相互作用不太明显。凝集素沉淀可以帮助结构研究识别，例如，末端糖单元和它们的异头键。
Alkali-treated lipopolysaccharides of a range of enterobacterial R mutants with complete and incomplete core structures were investigated by agar gel and microcapillary precipitation for reactivity with 3 selected commerical lectins, i.e., concanavalin A, Ricinus communis agglutinin and wheat germ agglutinin. R lipopolysaccharides with complete R cores of the 6 established core types were differentiated from each other by their characteristic lectin reactivity pattern. One core type showed no reaction with all 3 lectins. R lipopolysaccharides with incomplete core structures showed the reactivity pattern predictable from their individual chemical configuration. Substitution of complete R cores by haptens (T1, T2 or ECA) or by a single repeating unit (SR mutant) did not change the reactivity pattern, although some interactions were less pronounced. Lectin precipitation can be of help for structural studies in recognizing, e.g., terminal sugar units and their anomeric linkages.