[Experimental studies on the anti-thrombosis effect of 3,4-oxo-isopropylidene-shikimic acid].
[Experimental studies on the anti-thrombosis effect of 3,4-oxo-isopropylidene-shikimic acid].
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发表时间：
2002-04
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通讯作者：
Hong-tao Wang;H. Jin;Jian-ning Sun;Qiu-ping Xu;Ya-jian Guo
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作者：
Hong-tao Wang;H. Jin;Jian-ning Sun;Qiu-ping Xu;Ya-jian Guo
目的研究3，4-氧代-异亚丙基莽草酸(ISA)对大鼠动静脉分流和大脑中动脉血栓形成(MCAT)的影响。方法采用动静脉分流模型测定血栓重量，观察FeCl3诱导的大鼠中脑血栓形成(MCAT)模型的神经功能缺失(ND)和脑梗塞面积(IS)，观察ISA对大鼠和兔体内、外血小板聚集率的影响。结果ISA 25、50、100、200 mg·kg~(-1)ig可明显减轻动静脉分流模型血栓重量，ISA 50、100、200 mg·kg~(-1)ig 2次24 h可抑制MCAT大鼠的ND，ISA 100、200 mg·kg~(-1)可使MCAT大鼠的IS分别减少27.8%和31.6%，ISA 50、100、200 mg·kg~(-1)ig可抑制正常大鼠的血小板聚集，ISA 10(-3)~10(-5)mol.L~(-1)对兔体外血小板聚集有抑制作用。结论ISA通过抗血小板聚集抑制血栓形成。
AIM To study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) against arteriovenous shunt and middle cerebral artery thrombosis (MCAT) in rats. METHODS Arteriovenous shunt model was adopted to measure thrombus weight; The neurologic deficit (ND) and the infarct size (IS) of the middle cerebral thrombosis (MCAT) model induced by FeCl3 were observed; The effect of ISA on platelet aggregation rate of rat and rabbit by giving ISA in vivo and in vitro was studied. RESULTS ISA 25, 50, 100 and 200 mg.kg-1 ig was shown to reduced the weight of thrombus in arteriovenous shunt model; ISA 50, 100 and 200 mg.kg-1 ig for 2 times in 24 hours, attenuated the ND of rats subjected to MCAT; ISA 100 and 200 mg.kg-1 reduced IS of rats after MCAT by 27.8% and 31.6%, respectively; ISA 50, 100 and 200 mg.kg-1 ig inhibited platelet aggregation of normal rats; ISA 10(-3)-10(-5) mol.L-1, inhibited rabbit platelet aggregation in vitro. CONCLUSION ISA inhibited thrombosis by anti-platelet-aggregation.