Heterotypic cell interactions on a dually patterned surface
Heterotypic cell interactions on a dually patterned surface
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DOI：
10.1016/j.bbrc.2006.07.138
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发表时间：
2006-09-29
影响因子：
3.1
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Tsuda, Yukiko;Kikuchi, Akihiko;Okano, Teruo
通过模拟异型细胞在体内的结构和环境来研究它们之间的相互作用是值得的。本研究在一个新的共培养系统中，通过控制肝细胞和内皮细胞的接触外周，研究了肝细胞和内皮细胞的生理细胞反应和行为。大鼠原代肝细胞和牛内皮细胞在双图案表面上共培养。肝脏的生理功能，如白蛋白分泌和铵代谢，通过增加异型细胞-细胞相互作用在模式共培养增强。此外，肝细胞内白蛋白的免疫荧光染色证明，通过异型相互作用增强的肝功能在内皮细胞以外的有限区域内有效，表明异型相互作用以旁分泌方式起作用。因此，在增加肝脏生理功能中发挥不可或缺作用的异型细胞通讯应该通过增加双细胞结构域的外围来获得。这些发现对于肝脏、胰腺等复杂组织的重建具有重要意义。(c) 2006爱思唯尔公司版权所有。
It is worth investigating heterotypic cell-cell interactions by mimicking their in vivo structures and environment. In the present study, physiological cellular response and behavior of hepatocytes and endothelial cells were investigated by controlling their contact periphery in a new co-culture system. Rat primary hepatocytes and bovine endothelial cells were co-cultured on a dually patterned surface. Hepatic physiological functions such as albumin secretion and ammonium metabolism were enhanced by increasing heterotypic cell-cell interactions in a patterned co-culture. Furthermore, enhanced hepatic functions through heterotypic interactions are effective within a limited area apart from endothelial cells as evidenced by immunofluorescence staining of hepatic intracellular albumin, indicating that heterotypic interactions act in a paracrine manner. Thus, heterotypic cell communications that play indispensable roles in increasing hepatic physiological functions should be obtained with an increasing periphery of two-cell domains. These findings are important for the reconstruction of complex tissues such as liver and pancreas. (c) 2006 Elsevier Inc. All rights reserved.