A readily synthesized cyclic pyrrolysine analogue for site-specific protein "click" labeling
A readily synthesized cyclic pyrrolysine analogue for site-specific protein "click" labeling
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DOI：
10.1039/c1cc00024a
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Chen, Peng R.
中科院分区：
文献类型：
--
作者：
Hao, Ziyang;Song, Yanqun;Chen, Peng R.
开发了一条简洁的路线，用于轻松合成带有叠氮化物手柄的环状吡咯赖氨酸类似物。定向进化使得这种非天然氨基酸能够在原核和真核细胞中编码，这为使用点击化学进行位点特异性蛋白质标记提供了一种高效的方法。
A concise route was developed for the facile synthesis of a cyclic pyrrolysine analogue bearing an azide handle. Directed evolution enabled the encoding of this non-natural amino acid in both prokaryotic and eukaryotic cells, which offers a highly efficient approach for the site-specific protein labeling using click chemistry.