A panel of monoclonal antibodies against the prion protein proves that there is no prion protein in human pancreatic ductal epithelial cells
A panel of monoclonal antibodies against the prion protein proves that there is no prion protein in human pancreatic ductal epithelial cells
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DOI：
10.1007/s12250-014-3480-4
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发表时间：
2014-08-01
影响因子：
5.5
通讯作者：
Li, Chaoyang
中科院分区：
文献类型：
--
作者：
Yang, Liheng;Zhang, Yan;Li, Chaoyang
朊病毒病是一组致命的神经退行性疾病。在中国，对这些独特疾病的研究因缺乏资源而受到阻碍。生物学研究中最重要的资源是单克隆抗体。在这里，我们通过酶联免疫吸附试验（ELISA），免疫荧光染色，流式细胞术和蛋白质印迹法表征了一组细胞朊病毒蛋白特异性单克隆抗体。我们确定了几种抗体，可用于特定的应用程序，我们证明，有没有朊病毒蛋白在人胰腺导管上皮细胞（HPDC）的表达。
Prion diseases are a group of neurodegenerative diseases that are fatal. The study of these unique diseases in China is hampered by a lack of resources. Amongst the most important resources for biological study are monoclonal antibodies. Here, we characterize a panel of monoclonal antibodies specific for cellular prion protein by enzyme-linked immunosorbent assay (ELISA), immunofluorescent staining, flow cytometry, and western blotting. We identify several antibodies that can be used for specific applications and we demonstrate that there is no prion protein expression in human pancreatic ductal epithelial cells (HPDC).