Considerable haplotypic diversity in the RT1-CE class I gene region of the rat major histocompatibility complex
Considerable haplotypic diversity in the RT1-CE class I gene region of the rat major histocompatibility complex
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DOI：
10.1007/s00251-004-0744-4
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发表时间：
2005-01-01
期刊：
影响因子：
3.2
通讯作者：
Walter, L
中科院分区：
文献类型：
--
作者：
Roos, C;Walter, L
主要组织相容性复合体（MHC）的I类区域之间的蝙蝠1和Pou 5 f1基因的延伸表现出相当大的基因组可塑性在小鼠和恒河猴，但不是在人类单倍型。在大鼠中，该区域被称为RT 1-CE区域。最近发表的大鼠MHC序列在单个MHC单倍型中产生了一套完整的I类基因序列，即广泛使用的BN近交系的RT 1（n）单倍型。为了研究遗传多样性的程度，我们比较了RT 1-CE区域衍生的I类基因的RT 1 n单倍型与其他大鼠单倍型的I类序列。通过系统发育树分析，我们获得了证据，广泛的“存在和不存在”的单基因座，甚至小亚家族的I类基因在大鼠的多态性。RT 1-CE区的I类基因也可以被鉴定出等位基因，但等位基因核苷酸替换率似乎相当低，表明RT 1-CE区的多样性主要基于基因组可塑性。
The major histocompatibility complex (MHC) class I region extending between the Bat1 and Pou5f1 genes shows considerable genomic plasticity in mouse and rhesus macaque but not in human haplotypes. In the rat, this region is known as the RT1-CE region. The recently published rat MHC sequence gave rise to a complete set of class I gene sequences in a single MHC haplotype, namely the RT1(n) haplotype of the widely used BN inbred strain. To study the degree of genetic diversity, we compared the RT1-CE region-derived class I genes of the RT1n haplotype with class I sequences of other rat haplotypes. By using phylogenetic tree analyses, we obtained evidence for extensive "presence and absence" polymorphisms of single loci and even small subfamilies of class I genes in the rat. Alleles of RT1-CE region class I genes could also be identified, but the rate of allelic nucleotide substitutions appeared rather low, indicating that the diversity in the RT1-CE region is mainly based on genomic plasticity.