Statistical Analysis of Peptide Electron Transfer Dissociation Fragmentation Mass Spectrometry
Statistical Analysis of Peptide Electron Transfer Dissociation Fragmentation Mass Spectrometry
复制标题
DOI：
10.1021/ac9018582
复制
发表时间：
2010-01-15
影响因子：
7.4
通讯作者：
Burlingame, A. L.
中科院分区：
文献类型：
--
作者：
Chalkley, R. J.;Medzihradszky, K. F.;Burlingame, A. L.
已经确定，蛋白质序列测定可以通过碰撞活化的质子化Untie肽的质谱分析来实现。当通过纳米流HPLC分离时，通常可以从复杂的蛋白质混合物中鉴定出高百分比的肽。最近，替代的，自由基驱动的电子捕获解离和更常见的电子转移解离（ETD）的碎裂方法已被引入并广泛使用。为了在大规模蛋白质组学研究中利用这些技术，重要的是表征这些片段化过程对由一系列酶裂解形成的肽的性能。在这项研究中，我们提出了一个统计分析的离子类型，从不同的酶产生的肽，并强调ETD光谱的不同特点的双电荷前体相比，更高的电荷状态的前体。
It is well established that protein sequence determination may be achieved by mass spectrometric analysis of protonated Untie peptide subjected to collisional activation. When separated by nanoflow HPLC, a high percentage of peptides from complex mixtures of proteins can usually be identified. Recently, alternative, radical-driven fragmentation approaches of electron capture dissociation and the more common electron transfer dissociation (ETD) have been introduced and made widely available. In order to utilize these techniques in large scale proteomics studies, it is important to characterize the performance of these fragmentation processes on peptides formed by a range of enzymatic cleavages. In this study, we present a statistical analysis of the ion types that are observed from peptides produced by different enzymes and highlight the different characteristics of ETD spectra of doubly charged precursors in comparison to precursors of higher charge states.