Allelic variation at Fr-H1/Vrn-H1 and Fr-H2 loci is the main determinant of frost tolerance in spring barley
Allelic variation at Fr-H1/Vrn-H1 and Fr-H2 loci is the main determinant of frost tolerance in spring barley
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DOI：
10.1016/j.envexpbot.2014.02.014
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发表时间：
2014-10
影响因子：
5.7
通讯作者：
A. Tondelli;D. Pagani;Iman Naseh Ghafoori;M. Rahimi;R. Ataei;F. Rizza;Andrew J. Flavell;L. Cattivelli
中科院分区：
文献类型：
--
作者：
A. Tondelli;D. Pagani;Iman Naseh Ghafoori;M. Rahimi;R. Ataei;F. Rizza;Andrew J. Flavell;L. Cattivelli
抗冻性是谷类作物抗寒性的主要组成部分，并且在很大程度上取决于植物通过由低的非冻结温度诱导的缓慢适应性反应来适应冷的能力。在目前的工作中，全基因组关联作图研究已经进行，寻找耐霜冻的遗传决定因素在欧洲春季2行大麦品种的面板。通过测量叶绿素荧光（Fv/Fm）和膜完整性（电解质渗漏）来评估霜冻损害，并且发现该性状的相当大的变化。使用混合线性模型方法用>5000个基于基因的作图SNP标记寻找性状的遗传关联。一个简单的遗传结构揭示了该群体的抗冻性，与主要作用的Fr-H1/Vrn-H1和Fr-H2位点已经在以前的连锁和关联作图研究中检测到。等位基因的丰富性可能存在于这些位点，不仅在冬大麦和春大麦之间，而且在春种质内，从而为未来开发新的春大麦品系提供了机会，这些品系在面对新出现的气候变化时具有更好的弹性。
Frost tolerance is the main component of winter hardiness for cereals and is largely dependent on the plant's ability to cold acclimate by means of a slow adaptive response induced by low, non-freezing temperatures. In the present work, a Genome-Wide Association mapping study has been performed, looking for the genetic determinants of frost tolerance in a panel of European spring 2-row barley cultivars. Frost damage was evaluated by measuring chlorophyll fluorescence (Fv/Fm) and membrane integrity (Electrolyte Leakage) and considerable variation for this trait was discovered. Genetic association of the trait was sought with >5000 gene-based mapped SNP markers using a Mixed Linear Model approach. A simple genetic architecture was revealed for frost tolerance in this population, with a principal role for theFr-H1/Vrn-H1andFr-H2loci already detected in previous linkage and association mapping studies. Allelic richness might exist at these loci, not only between winter and spring barley, but also within the spring germplasm, thus offering the opportunity for future development of new spring barley lines with improved resilience in the face of emerging climate change.