Presence of multiple species of polypeptides immunologically related to transcription factor TFIIIA in adult Xenopus tissues.
Presence of multiple species of polypeptides immunologically related to transcription factor TFIIIA in adult Xenopus tissues.
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成年非洲爪蟾组织中存在多种与转录因子 TFIIIA 免疫相关的多肽。
DOI：
10.1093/nar/17.14.5597
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发表时间：
1989
影响因子：
14.9
通讯作者：
Ryoji,M
中科院分区：
文献类型：
--
作者：
Yasui,W;Ryoji,M
在非洲爪哇卵母细胞中转录5S RNA基因需要一个38 kDa的转录因子TFIIIA，该转录因子与该基因50bp的内控区相互作用。根据TFIIIA样多肽与抗TFIHA抗体的抗原交叉反应，我们在成虫组织提取物中寻找TFIIIA样多肽。在肾脏、胃、肝脏和睾丸中发现了几种大小在30-50 kDa之间的多肽。尽管这些多肽与抗TFIIIA抗体发生特异性反应，但三个代表性多肽的蛋白水解肽图谱并没有显示出任何相似之处，它们似乎也与TFIIIA不同。文中还讨论了这些蛋白的可能功能。
Transcription of5S RNA gene in Xenopus oocytesrequires a 38kDa transcription factor TFIIIA,which interacts with the 50 bp internal control region of the gene. We looked for TFIIIA-like polypeptides in the extracts of adultXenopustissues on the basis of their antigenic cross-reactivity to anti-TFIHA antibody. Several species of polypeptides ranging from 30 to 50 kDa were found in kidney, stomach, liver and testis. Although these polypeptides reacted specifically to anti-TFIIIA antibody, proteolytic peptide mapping of three representative ones did not reveal any mutual similarities.They also seemed to be distinct from TFIIIA. Possible functions of these proteins are discussed.