Bone marrow cells repair cigarette smoke-induced emphysema in rats
Bone marrow cells repair cigarette smoke-induced emphysema in rats
复制标题
DOI：
10.1152/ajplung.00253.2010
复制
发表时间：
2011-09-01
影响因子：
4.9
通讯作者：
Lee, Sang-Do
中科院分区：
文献类型：
--
作者：
Huh, Jin Won;Kim, Sun-Yong;Lee, Sang-Do
Huh JW，Kim S-Y，Lee JH，Lee J-S，货车Ta Q，Kim M，Oh Y-M，Lee Y-S，Lee S-D.骨髓细胞修复香烟烟雾诱导的大鼠肺气肿。美国生理学杂志肺细胞分子生理学301：L255-L266，2011年。首次发表于2011年5月27日; doi：10.1152/ajplung.00253.2010.-尽管干细胞疗法在其他肺部疾病模型中是有效的，但干细胞在慢性阻塞性肺疾病中的治疗潜力还不为人所知。我们检测了骨髓细胞（BMCs）、间充质干细胞（MSCs）和MSCs条件培养基（MSC-CM）修复香烟烟雾诱导的肺气肿的能力。将近交系雌性刘易斯大鼠暴露于香烟烟雾中6个月，然后接受来自雄性刘易斯大鼠的BMCs、MSC或MSC-CM。注射后2个月，BMC治疗逐渐减轻香烟烟雾诱导的肺气肿，并恢复增加的平均线性截距。BMC治疗显著增加细胞增殖和肺小血管的数量，减少凋亡细胞死亡，减弱平均肺动脉压，并抑制肺小血管的肌化。然而，只有少数男性供体细胞检测到BMC给药后1天至1个月。MSC和无细胞MSC-CM还诱导肺气肿的修复，并增加肺小血管的数量。我们的数据显示，BMC、MSC和MSC-CM治疗修复了香烟烟雾诱导的肺气肿。这些治疗的修复活性与旁分泌效应而不是干细胞植入一致，因为大多数供体细胞消失，并且因为无细胞MSC-CM也诱导了修复。
Huh JW, Kim S-Y, Lee JH, Lee J-S, Van Ta Q, Kim M, Oh Y-M, Lee Y-S, Lee S-D. Bone marrow cells repair cigarette smoke-induced emphysema in rats. Am J Physiol Lung Cell Mol Physiol 301: L255-L266, 2011. First published May 27, 2011; doi:10.1152/ajplung.00253.2010.-The therapeutic potential of stem cells in chronic obstructive pulmonary disease is not well known although stem cell therapy is effective in models of other pulmonary diseases. We tested the capacities of bone marrow cells (BMCs), mesenchymal stem cells (MSCs), and conditioned media of MSCs (MSC-CM) to repair cigarette smoke-induced emphysema. Inbred female Lewis rats were exposed to cigarette smoke for 6 mo and then received BMCs, MSCs, or MSC-CM from male Lewis rats. For 2 mo after injection, the BMC treatment gradually alleviated the cigarette smoke-induced emphysema and restored the increased mean linear intercept. The BMC treatment significantly increased cell proliferation and the number of small pulmonary vessels, reduced apoptotic cell death, attenuated the mean pulmonary arterial pressure, and inhibited muscularization in small pulmonary vessels. However, only a few male donor cells were detected from 1 day to 1 mo after BMC administration. The MSCs and cell-free MSC-CM also induced the repair of emphysema and increased the number of small pulmonary vessels. Our data show that BMC, MSCs, and MSC-CM treatment repaired cigarette smoke-induced emphysema. The repair activity of these treatments is consistent with a paracrine effect rather than stem cell engraftment because most of the donor cells disappeared and because cell-free MSC-CM also induced the repair.