A single HBsAg DNA vaccination in combination with electroporation elicits long-term antibody responses in sheep
A single HBsAg DNA vaccination in combination with electroporation elicits long-term antibody responses in sheep
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DOI：
10.1016/j.bioelechem.2006.10.003
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发表时间：
2007-05-01
影响因子：
5
通讯作者：
Griebel, Philip J.
中科院分区：
文献类型：
--
作者：
Babiuk, Shawn;Tsang, Cemaine;Griebel, Philip J.
疫苗仍然是减少人类和动物健康疾病负担的最具成本效益的方法。然而，需要改善疫苗接种后的免疫持续时间，因为如果疫苗要提供长期保护性免疫，则维持血清中抗体的保护性水平或在再次暴露时快速应答的能力（记忆）是至关重要的。本实验的目的是测试通过多种途径递送的编码B型肝炎表面抗原（HBsAg）的DNA疫苗单次免疫后抗体应答的持续时间和产生回忆应答的能力。用常规HBsAg亚单位疫苗（Engerix-B）免疫的绵羊以及用HBsAg DNA疫苗免疫的绵羊，结合电穿孔，产生显著的抗体应答，在初次免疫后持续25周。在25周时，所有实验组均接受HBsAg亚单位疫苗的二次免疫。接受初次DNA免疫的绵羊，结合电穿孔，建立了与HBsAg亚单位疫苗免疫的队列相似的回忆应答。相反，用DNA疫苗免疫的动物在没有电穿孔的情况下没有引起可检测的记忆反应。免疫记忆的存在与诱导延长的初次免疫应答显著相关。因此，一个单一的DNA疫苗接种，结合电穿孔，接近商业亚单位疫苗在长期保护性血清抗体滴度和免疫记忆的维持的功效。(C)2006 Elsevier B. V.保留所有权利。
Vaccines continue to be the most cost effective method to reduce the burden of disease in both human and animal health. However, there is a need to improve the duration of immunity following vaccination, since maintenance of protective levels of antibody in serum or the ability to rapidly respond upon re-exposure (memory) is critical if vaccines are to provide long-term protective immunity. The purpose of this experiment was to test the duration of antibody responses and the ability to generate anamnestic responses following a single immunization with a DNA vaccine encoding hepatitis B surface antigen (HBsAg) delivered by a variety of routes. Sheep immunized with the conventional HBsAg subunit vaccine (Engerix-B) as well as sheep immunized with a HBsAg DNA vaccine, combined with electroporation, generated significant antibody responses that were sustained for 25 weeks after primary immunization. At 25 weeks, all experimental groups received a secondary immunization with the HBsAg subunit vaccine. Sheep that received a primary DNA immunization, in combination with electroporation, mounted an anamnestic response similar to the cohort immunized with the HBsAg subunit vaccine. In contrast, animals immunized with DNA vaccines administered without electroporation elicited no detectable memory response. The presence of immune memory was significantly correlated with the induction of a prolonged primary immune response. Thus, a single DNA vaccination, in combination with electroporation, approached the efficacy of the commercial subunit vaccine in the maintenance of long-term protective serum antibody titres and immune memory. (C) 2006 Elsevier B.V. All rights reserved.