Targeted lymph node immunization with simian immunodeficiency virus p27 antigen to elicit genital, rectal, and urinary immune responses in nonhuman primates.
Targeted lymph node immunization with simian immunodeficiency virus p27 antigen to elicit genital, rectal, and urinary immune responses in nonhuman primates.
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使用猿猴免疫缺陷病毒 p27 抗原进行靶向淋巴结免疫，以引发非人灵长类动物的生殖器、直肠和泌尿道免疫反应。
作者：
Thomas Lehner;L. Bergmeier;L. Tao;C. Panagiotidi;L. Klavinskis;L. Hussain;R. Ward;N. Meyers;Sally E. Adams;A. Gearing
皮下注射在非人灵长类动物中开发了免疫途径，其靶向生殖器-直肠相关淋巴组织。在髂内淋巴结附近施用由表达为杂合Ty病毒样颗粒（p27：Ty-VLP）的rSIV gag p27组成的疫苗。在阴道、男性尿道、直肠和精液、尿液和血清中均检测到p27抗原的分泌型伊加和IgG抗体。使用血清或胆汁伊加和IgG Ab，在p27序列的肽51-90和121-170内鉴定出两个或多个免疫显性B细胞表位。CD 4 + T细胞对p27的增殖反应主要在靶向髂内、肠系膜下淋巴结和脾脏中引起，但在无关淋巴结中不引起。然后研究这些细胞在p27特异性B细胞Ab合成中的辅助功能。特异性伊加和IgG抗体检测到在相同的淋巴组织，显示增殖反应。然而，脾和髂淋巴结B和CD 4 + T细胞之间的交叉重建实验表明，髂B细胞对于特异性伊加Ab合成是必需的，而脾B细胞优先合成IgG Ab。靶向淋巴结（TLN）免疫途径与阴道、男性泌尿生殖道和直肠粘膜免疫途径相比，B细胞、增殖性T细胞和Th细胞反应相当，口服免疫可增强这些反应。然而，除了生殖器和直肠Ab外，TLN途径还诱导尿液和精液sIgA和IgG Ab。通过TLN免疫在粘膜表面产生分泌型伊加和IgG Ab，以及在区域引流淋巴结、脾和循环中产生T和B细胞免疫，可以防止病毒通过粘膜传播、病毒传播和形成潜伏感染库。
A s.c. route of immunization was developed in non-human primates, which targets the genitourinary-rectal associated lymphoid tissue. A vaccine consisting of rSIV gag p27, expressed as hybrid Ty virus-like particles (p27: Ty-VLP) was administered in the proximity of the internal iliac lymph nodes. Secretory IgA and IgG Abs to the p27 Ag were elicited in the vaginal, male urethral, rectal and seminal fluids, urine and serum. Two or more immunodominant B cell epitopes were identified within peptides 51-90 and 121-170 of the sequence of p27, using serum or biliary IgA and IgG Abs. CD4+ T cell proliferative responses to p27 were elicited predominantly in the targeted internal iliac, as well as the inferior mesenteric lymph nodes and the spleen, but not in the unrelated lymph nodes. These cells were then studied for helper function in p27 specific B cell Ab synthesis. Specific IgA and IgG Abs were detected in the same lymphoid tissues as those that displayed proliferative responses. However, cross-over reconstitution experiments between splenic and iliac lymph node B and CD4+ T cells suggest that the iliac B cells are essential for specific IgA Ab synthesis, whereas splenic B cells preferentially synthesize IgG Ab. The targeted lymph node (TLN) route of immunization gave comparable B cell, proliferative T cell, and Th cell responses to the vaginal, male genitourinary, and rectal mucosal routes, which were augmented by oral immunization. However, the TLN route induced urinary and seminal fluid sIgA and IgG Abs in addition to genital and rectal Abs. Generating secretory IgA and IgG Abs at the mucosal surfaces, and T and B cell immunity in the regional draining lymph nodes, spleen and circulation by TLN immunization may prevent transmission of virus through the mucosa, dissemination of the virus, and the formation of a latent reservoir of infection.
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盆腔、肠胃外或阴道免疫后小鼠阴道液中的分泌性免疫反应。
影响因子：
6.4
作者：
Thapar,MA;Parr,EL;Parr,MB
通讯作者：
Parr,MB
DOI：
10.1073/pnas.89.15.6901
发表时间：
1992-08-01
影响因子：
11.1
作者：
MAZANEC, MB;KAETZEL, CS;NEDRUD, JG
通讯作者：
NEDRUD, JG
CH 同种型在正常 B 淋巴细胞发育过程中“转换”。
影响因子：
29.7
作者：
Cebra,JJ;Komisar,JL;Schweitzer,PA
通讯作者：
Schweitzer,PA
女性生殖道分泌物中的特异性 IgA 和 IgG 抗体：免疫和雌二醇对体内这种反应表达的影响。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Wira,CR;Sandoe,CP
通讯作者：
Sandoe,CP