MEMBRANE-ASSOCIATED CARBONIC-ANHYDRASE IN GILLS OF THE BLUE-CRAB, CALLINECTES-SAPIDUS
MEMBRANE-ASSOCIATED CARBONIC-ANHYDRASE IN GILLS OF THE BLUE-CRAB, CALLINECTES-SAPIDUS
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DOI：
10.1152/ajpregu.1987.252.5.r966
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发表时间：
1987-05-01
影响因子：
--
通讯作者：
HENRY, RP
中科院分区：
文献类型：
--
作者：
HENRY, RP
用一种膜不透性的碳酸酐酶抑制剂--季铵磺胺（QAS），在活体条件下研究了青蟹鳃内皮细胞基底膜上碳酸酐酶（CA）的存在及其生理意义。将QAS注射到Callinectes sapidus的血淋巴中导致呼吸性酸中毒的快速发展; PCO 2上升几乎2托，pH降低约0.25单位，和总CO2上升了2毫米。这些结果支持的假设，膜相关的CA暴露于血淋巴是存在于甲壳动物鳃，它是生理上重要的动员血淋巴HCO 3-CO2，以促进CO2排泄整个鳃。这种酸中毒的恢复与抑制剂从血淋巴中的清除一致。血淋巴渗透和离子参数不受QAS，再次确认鳃胞质CA在离子调节中的作用，也提供了一个方便的生物测定确定CA抑制剂的渗透性在完整的有机体。
The presence of the enzyme carbonic anhydrase (CA) on the basal membrane of the branchial endothelial cells in the blue crab and its physiological significance were studied in vivo using a membrane-impermeant CA inhibitor, quaternary ammonium sulfanilamide (QAS). Injection of QAS into the hemolymph of Callinectes sapidus resulted in the rapid development of a respiratory acidosis; PCO2 rose almost 2 Torr, pH was lowered by .apprx. 0.25 units, and total CO2 rose by 2 mM. These results support the hypothesis that membrane-associated CA exposed to hemolymph is present in the crustacean gill and that it is physiologically significant in mobilizing hemolymph HCO3- to CO2 to facilitate CO2 excretion across the gill. The recovery from this acidosis coincides with the clearance of the inhibitor from the hemolymph. Hemolymph osmotic and ionic parameters were unaffected by QAS, reconfirming the role of branchial cytoplasmic CA in ion regulation and also providing a convenient bioassay for determining CA inhibitor permeability in the intact organism.