Silicon chips detect intracellular pressure changes in living cells
Silicon chips detect intracellular pressure changes in living cells
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DOI：
10.1038/nnano.2013.118
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发表时间：
2013-07-01
影响因子：
38.3
通讯作者：
Plaza, Jose A.
中科院分区：
文献类型：
--
作者：
Gomez-Martinez, Rodrigo;Hernandez-Pinto, Alberto M.;Plaza, Jose A.
测量活细胞不同成分内部压力变化的能力很重要，因为它为研究涉及细胞变形的基本过程提供了另一种方法。大多数目前的技术，如吸移管(2)、光学干涉法(3)或外部压力探头(4)，要么使用间接测量方法，要么使用可能损害细胞膜的方法。在这里，我们展示了一个小到可以内化到活细胞中的硅芯片可以用来检测细胞内的压力变化。该芯片由两个由真空间隙隔开的薄膜组成，形成法布里-珀罗谐振器，它检测压力变化，可以根据反射光的强度进行量化。利用这个芯片，我们证明了细胞外的静水压力被传递到HeLa细胞中，这些细胞可以承受低渗透压力，而不会显著增加细胞内的静水压力。
The ability to measure pressure changes inside different components of a living cell is important, because it offers an alternative way to study fundamental processes that involve cell deformation(1). Most current techniques such as pipette aspiration(2), optical interferometry(3) or external pressure probes(4) use either indirect measurement methods or approaches that can damage the cell membrane. Here we show that a silicon chip small enough to be internalized into a living cell can be used to detect pressure changes inside the cell. The chip, which consists of two membranes separated by a vacuum gap to form a Fabry-Perot resonator, detects pressure changes that can be quantified from the intensity of the reflected light. Using this chip, we show that extracellular hydrostatic pressure is transmitted into HeLa cells and that these cells can endure hypo-osmotic stress without significantly increasing their intracellular hydrostatic pressure.