Community structure and transcript responses of anammox bacteria, AOA, and AOB in mangrove sediment microcosms amended with ammonium and nitrite
Community structure and transcript responses of anammox bacteria, AOA, and AOB in mangrove sediment microcosms amended with ammonium and nitrite
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DOI：
10.1007/s00253-012-4683-y
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发表时间：
2013-11-01
影响因子：
5
通讯作者：
Gu, Ji-Dong
中科院分区：
文献类型：
--
作者：
Li, Meng;Gu, Ji-Dong
人为氮输入是影响海岸带生态系统微生物氮循环的重要来源。以红树林沉积物为研究对象，通过室内微宇宙培养，研究了厌氧氨氧化细菌、氨氧化古菌（AOA）和氨氧化细菌（AOB）对氨氮和亚硝酸盐的响应。在培养过程中监测了厌氧氨氧化菌16 SrRNA和肼氧化还原酶（hzo）基因以及AOA和AOB的amoA基因的多样性、丰度和转录的变化。T-RFLP分析表明，铵和亚硝酸盐的添加显著改变了厌氧氨氧化细菌、AOA和AOB的群落组成，而丰度和转录本定量分析证实，铵（25 mM）的添加刺激了厌氧氨氧化细菌、AOA和AOB的生长，而亚硝酸盐则刺激了厌氧氨氧化细菌、AOA和AOB的生长。（0.8 mM）一般抑制他们与AOA和AOB的特定物种的一些例外，表现出不同的反应的厌氧氨氧化细菌，AOA，和AOB的亚硝酸盐和铵的修正案。结果进一步表明，AOB作为优势类群，其amoA基因丰度和转录本含量较高，可能在该亚热带地区红树林沉积物中氨氧化过程中起着重要作用。
The anthropogenic nitrogen (N) input as an important source strongly influences the microbial N cycling in coastal ecosystems. In this study, we investigated the responses of anammox bacteria, ammonia oxidizing archaea (AOA), and ammonia oxidizing bacteria (AOB) to the amendments of ammonium and nitrite into mangrove sediments incubated in laboratory microcosm experiments. The variations of diversity, abundances, and transcription of 16S rRNA and hydrazine oxidoreductase (hzo) genes for anammox bacteria, and amoA genes for AOA and AOB were monitored during the incubation. The T-RFLP analysis demonstrated that both ammonium and nitrite additions significantly altered the community compositions of anammox bacteria, AOA, and AOB, while abundance and transcripts analyzed quantitatively confirmed that the amendment of ammonium (25 mM) stimulated the growth of anammox bacteria, AOA, and AOB, whereas nitrite (0.8 mM) generally inhibited them with some exceptions for specific species of AOA and AOB, showing different responses of anammox bacteria, AOA, and AOB to the nitrite and ammonium amendments. Results further suggest that AOB as the dominant group with higher amoA gene abundances and transcripts might play a more important role on the ammonium oxidization in mangrove sediment of this subtropical site.