Adherence of microorganisms to rat salivary pellicles.
Adherence of microorganisms to rat salivary pellicles.
复制标题
微生物对大鼠唾液膜的粘附。
DOI：
10.1159/000262122
复制
发表时间：
1995
期刊：
影响因子：
4.2
通讯作者：
Bowen,WH
中科院分区：
文献类型：
--
作者：
Kopec,LK;Bowen,WH
许多研究使用模型来模拟获得性膜的形成，集中在人类唾液包裹的羟基磷灰石上；相反，尽管大鼠经常被用作动物模型，但关于大鼠唾液形成膜的信息很少。我们比较了链球菌和放线菌对大鼠唾液包被的羟基磷灰石(RsHA)和人唾液包被的羟基磷灰石(Sa)的附着能力。我们还探讨了原位合成的葡聚糖对生物黏附的影响。我们表明，每个生物体菌株都有不同的结合模式，这在本质上是相同的，表面涂有老鼠或人的唾液。溶解的重组血凝素微球显示一条主要的蛋白质带(35-19kD)，经鉴定为谷氨酰胺/谷氨酸富含蛋白，酸性富含脯氨酸蛋白(31-45kD)和α-淀粉酶(66kD)。总体而言，这些体外数据强烈表明细菌对rsHA的黏附原理与SHA观察到的相似。
Numerous studies, using models to mimic the formation of the acquired pellicle, have concentrated on human saliva-coated hydroxyapatite; in contrast, although the rat is frequently used as an animal model, the information concerning the formation of pellicles from rat saliva is sparse. We compared the ability ofStreptococcusandActinomycesspecies to adhere to hydroxyapatite coated by rat saliva (rsHA) and human saliva (sHA). We also explored the influence of glucan synthesized in situ on the adherence of organisms. We show that each strain of organism has a distinct binding pattern, which was essentially the same with surfaces coated with either rat or human saliva. Dissolved rsHA beads revealed a major protein band (35–19 kD), identified as glutamine/glutamic-acid-rich protein; acidic proline-rich protein (31–45 kD and α-amylase (66 kD) were also identified. Overall, these in vitro data strongly suggest that the principles of bacterial adhesion to rsHA are similar to those observed with sHA.