Nucleosome fragility reveals novel functional states of chromatin and poises genes for activation
Nucleosome fragility reveals novel functional states of chromatin and poises genes for activation
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DOI：
10.1101/gr.117101.110
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发表时间：
2011-05-01
期刊：
影响因子：
7
通讯作者：
He, Xiangwei
中科院分区：
文献类型：
--
作者：
Xi, Yuanxin;Yao, Jianhui;He, Xiangwei
核小体的结构复杂性是其功能多样性的基础。在这里，我们报告了一种新型的复杂性核小体脆性，表现为高敏感性微球菌核酸酶，在相反的共同假设，核小体是类似的耐MNase消化。使用差异MNase消化染色质和高通量测序，我们已经确定了一组特殊的核小体称为“脆弱核小体”在整个酵母基因组中，其中近1000个是在以前确定的“无核小体”位点。核小体脆性广泛涉及多个染色质过程，包括转录、易位和复制，与细胞的特定生理状态相对应。在环境胁迫反应基因中，在环境变化发生之前存在脆弱的核小体，表明核小体的脆弱性使基因响应环境变化而迅速上调。我们认为核小体的脆弱性强调了染色质的不同功能状态，并为描绘基因组组织的景观提供了一个新的维度。
The structural complexity of nucleosomes underlies their functional versatility. Here we report a new type of complexity-nucleosome fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumption that nucleosomes are similar in resistance to MNase digestion. Using differential MNase digestion of chromatin and high-throughput sequencing, we have identified a special group of nucleosomes termed "fragile nucleosomes'' throughout the yeast genome, nearly 1000 of which were at previously determined "nucleosome-free'' loci. Nucleosome fragility is broadly implicated in multiple chromatin processes, including transcription, translocation, and replication, in correspondence to specific physiological states of cells. In the environmental-stress-response genes, the presence of fragile nucleosomes prior to the occurrence of environmental changes suggests that nucleosome fragility poises genes for swift up-regulation in response to the environmental changes. We propose that nucleosome fragility underscores distinct functional statuses of the chromatin and provides a new dimension for portraying the landscape of genome organization.