Effects of connective tissue growth factor on human periodontal ligament fibroblasts
Effects of connective tissue growth factor on human periodontal ligament fibroblasts
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DOI：
10.1016/j.archoralbio.2017.09.010
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发表时间：
2017-12-01
影响因子：
3
通讯作者：
Lin, Zhiyong
中科院分区：
文献类型：
--
作者：
Duan, Xuejing;Ji, Mei;Lin, Zhiyong
目的：观察不同浓度结缔组织生长因子(CTGF)对人牙周膜成纤维细胞(HPLF)的影响。然后，将不同浓度的CTGF(1、5、10、50、100 ng/ml)加入HPLF培养中。采用CCK-8比色法、碱性磷酸酶(ALP)比色法、羟脯氨酸测定法、茜素红染色法、Transwell小室和实时荧光定量聚合酶链式反应(Real-time PCR)等方法，观察CTGF对细胞增殖、碱性磷酸酶(ALP)活性、胶原合成、矿化结节形成和迁移的影响。我们还研究了碱性磷酸酶(ALP)、纤维连接蛋白(FN)、整合素结合唾液酸蛋白(IBSP)、骨钙素(OC)和整合素β1(ITGB1)的mRNA在HPLF中的表达。结果：CTGF(1、5、10 ng/ml)可显著促进HPLF的增殖和胶原合成。CTGF(1、5、10、50 ng/ml)可提高HPLF的ALP活性，而在所有浓度下，CTGF(1、5、10、50、100 ng/ml)可促进ALP、FN、IBSP和ITGB1mRNA的表达。此外，CTGF(1、5、10、50、100 ng/ml)可促进HPLF的迁移，且呈剂量依赖关系，其中以10 ng/ml组的促进作用最大(P<；0.05或P<；0.01)。结论：在一定浓度范围内，CTGF可促进HPLF的增殖、迁移和胶原合成等生物学效应，促进HPLF在成骨过程中的分化。
Objective: The aim of this study was to evaluate the effects of different concentrations of connective tissue growth factor (CTGF) on human periodontal ligament fibroblasts(HPLFs).Design: HPLFs were cultured and identified. Then, different concentrations of CTGF (1, 5, 10, 50, 100 ng/ml) were added to the HPLF culture. Next, CCK-8 assays, alkaline phosphatase (ALP) assays, hydroxyproline determination, alizarin red staining methods, Transwell chambers and real-time PCR methods were applied to observe the effects of CTGF on the proliferation, ALP activity, synthesis of collagen, formation of mineralized nodules and migration. We also studied expression of ALP, fiber link protein (FN), integrin-binding sialoprotein (IBSP), osteocalcin (OC), and integrin beta 1 (ITGB1) mRNA by HPLFs. Statistical significance was assumed if P < 0.05 or P < 0.01.Results: The addition of CTGF (1, 5, 10 ng/ml) remarkably promoted the proliferation and collagen synthesis of HPLFs compared with controls. CTGF (1, 5, 10, 50 ng/ml) improved ALP activity of HPLFs, and at all concentrations, CTGF (1, 5, 10, 50, 100 ng/ml) improved the expression of ALP, FN, IBSP and ITGB1 mRNA. In addition, CTGF (1, 5, 10, 50, 100 ng/ml) promoted the migration of HPLFs, which was dose-dependent, with maximal promotion in the 10 ng/ml group (P < 0.05 or P < 0.01).Conclusions: Thus, in a certain range of concentrations, CTGF can promote the biological effects, including proliferation, migration and collagen synthesis of HPLFs, to promote the differentiation of HPLFs in the process of osteogenesis.