Spatial organization of Myxococcus xanthus during fruiting body formation
Spatial organization of Myxococcus xanthus during fruiting body formation
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DOI：
10.1128/jb.01008-07
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发表时间：
2007-12-01
影响因子：
3.2
通讯作者：
Shimkets, Lawrence J.
中科院分区：
文献类型：
--
作者：
Curtis, Patrick D.;Taylor, Rion G.;Shimkets, Lawrence J.
利用显微摄影技术对黄色粘球菌子实体的发育过程进行了研究。野生型细胞的进展通过三个不同的阶段：一个静止期与一些运动，但很少聚集（0至8小时），一个时期的蓬勃运动导致提高子实体（8至16小时），和一个时期的成熟，在此期间，孢子形成开始（16至48 111）。子实体在一系列层中垂直延伸，每个层涉及在最上层的顶部上添加细胞单层。pilA（MXAN_5783）突变体产生较少的细胞外基质材料，因此允许更仔细地检查分层聚集体形成。一个csgA（MXAN_1294）突变体表现出没有静止期，异常聚集的阶段2，和解体的子实体在第三阶段。
Microcinematography was used to examine fruiting body development of Myxococcus xanthus. Wild-type cells progress through three distinct phases: a quiescent phase with some motility but little aggregation (0 to 8 h), a period of vigorous motility leading to raised fruiting bodies (8 to 16 h), and a period of maturation during which sporulation is initiated (16 to 48 111). Fruiting bodies are extended vertically in a series of tiers, each involving the addition of a cell monolayer on top of the uppermost layer. A pilA (MXAN_5783) mutant produced less extracellular matrix material and thus allowed closer examination of tiered aggregate formation. A csgA (MXAN_1294) mutant exhibited no quiescent phase, aberrant aggregation in phase 2, and disintegration of the fruiting bodies in the third phase.