Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity
Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity
复制标题
DOI：
10.2527/jas.2009-2583
复制
发表时间：
2010-04-01
影响因子：
3.3
通讯作者：
Drevet, J. R.
中科院分区：
文献类型：
--
作者：
Chabory, E.;Damon, C.;Drevet, J. R.
在哺乳动物中，睾丸后附睾精子成熟被认为是未成熟睾丸配子向能够受精的成熟精子转化的重要步骤。活性氧（Reactive oxygen species， ROS）在精子成熟过程中起着关键作用，现在已经很清楚，在精子成熟和获能等过程中，ROS是精子生理学的核心。然而，在附睾成熟和储存过程中，直到受精开始，氧化损伤是精子比任何其他细胞更必须面对的威胁。研究发现，精子对与脂质过氧化、DNA损伤和精子活力受损相关的氧化攻击极其敏感，所有这些都会影响受精。为了控制雄性配子附近H2O2的数量，哺乳动物附睾使用了一组非酶和酶清除剂，其中谷胱甘肽过氧化物酶（GPx）家族具有很大的代表性。在哺乳动物附睾表达的多种GPx蛋白中，GPx4和GPx5占据着独特的位置和功能，本文对此进行综述。本文强调了GPx蛋白家族在决定哺乳动物精子受精率方面的重要性。这在哺乳动物生育和不育领域以及辅助医疗生殖技术和男性配子保存技术的发展中尤其相关，这些技术广泛应用于人类和动物的生殖计划中。
In mammals, posttesticular epididymal sperm maturation is considered an essential step in the transformation of immature testicular gametes to mature spermatozoa capable of fertilization. Reactive oxygen species (ROS) have been shown to be key actors in this maturation process, and it is now clear that ROS are central for sperm physiology in processes such as sperm maturation and capacitation. However, during epididymal maturation and storage and until the onset of fertilization, oxidative damage is a threat spermatozoa must face more than any other cells. Spermatozoa were found to be extremely sensitive to oxidative attacks correlated with lipid peroxidation, DNA damage, and impaired sperm motility, all affecting fertilization. To control the quantity of H2O2 in the vicinity of male gametes, mammalian epididymis uses a panel of nonenzymatic and enzymatic scavengers, among which the glutathione peroxidase (GPx) family is largely represented. Among the various GPx proteins expressed in the mammalian epididymis, GPx4 and GPx5 occupy unique positions and functions that are reviewed in this paper. This paper underlines the importance of the GPx protein family in determining the fertilizing potential of mammalian spermatozoa. This is particularly relevant in the field of mammalian fertility and infertility as well as in the development of assisted medical procreation technologies and male gamete preservation techniques that are extensively used in human and animal reproduction programs.