KINETICS OF THE INITIAL ROUNDS OF CELL DIVISION OF TOXOPLASMA GONDII
KINETICS OF THE INITIAL ROUNDS OF CELL DIVISION OF TOXOPLASMA GONDII
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弓形虫细胞分裂最初几轮的动力学
DOI：
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发表时间：
2003
影响因子：
1.3
通讯作者：
D. Woodmansee
中科院分区：
文献类型：
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作者：
D. Woodmansee
RH株弓形虫速殖子，自然裂解其宿主细胞，允许感染新的宿主细胞有限的时间，随后的寄生虫细胞分裂密切观察。基于4项独立试验，估计的首次细胞分裂时间为感染后9.8小时（PI），并且变化很大（95%置信区间[CI]：3.1-16.5小时PI）。第二次细胞分裂的估计时间为PI 14.9小时，变化较小（95% CI：PI 12.1-17.7小时）。在这4项试验中，很少有寄生虫在PI 6小时前分裂。当速殖子通过强制裂解（刮取感染的宿主细胞培养物并将其通过27号针头）获得时，第一次寄生虫细胞分裂发生得比任何通过自然裂解获得的寄生虫试验中观察到的要快得多。当通过强制裂解衍生的寄生虫远离宿主细胞3小时PI时，第一次细胞分裂以类似于通过自然裂解衍生的寄生虫中所见的方式延迟。没有观察到任何差异的寄生虫的第二次细胞分裂的时间通过强制裂解是否已举行远离细胞。这些研究表明，速殖子在从一个宿主细胞到另一个宿主细胞的转运过程中暴露的条件可以影响寄生虫细胞在新宿主细胞中分裂的动力学。
RH strain Toxoplasma gondii tachyzoites that had naturally lysed their host cells were allowed to infect new host cells for a limited amount of time; subsequent parasite cell divisions were observed closely. On the basis of 4 independent trials, the estimated time to first cell division was 9.8 hr postinfection (PI) and was quite variable (95% confidence interval [CI]: 3.1–16.5 hr PI). The estimated time to second cell division was 14.9 hr PI and was less variable (95% CI: 12.1–17.7 hr PI). Few parasites divided before 6 hr PI in these 4 trials. When tachyzoites were derived by forced lysis (scraping an infected host cell culture and passing it through 27-gauge needles), the first parasite cell division occurred much more rapidly than had been observed in any of the trials with parasites derived by natural lysis. When parasites derived by forced lysis were held away from host cells for 3 hr PI, the first cell division was delayed in a manner similar to that seen in parasites derived by natural lysis. No differences were observed in the timing of the second cell division of parasites derived by forced lysis whether or not they had been held away from cells. These studies demonstrate that the conditions to which tachyzoites are exposed during transit from one host cell to another can affect the kinetics of parasite cell division in the new host cell.
微管的破坏使弓形虫的出芽和核分裂脱钩。
影响因子：
4
作者：
Morrissette,NaomiS;Sibley,LDavid
通讯作者：
Sibley,LDavid