Synthesis of biotinylated sialoside to probe CD22-ligand interactions
Synthesis of biotinylated sialoside to probe CD22-ligand interactions
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DOI：
10.1016/j.tetlet.2009.05.044
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发表时间：
2009-08-05
影响因子：
1.8
通讯作者：
Kiso, Makoto
中科院分区：
文献类型：
--
作者：
Abdu-Allah, Hajjaj H. M.;Watanabe, Kozo;Kiso, Makoto
设计并合成了小鼠CD_（22）最强配体生物素偶联物。关键的合成步骤涉及Hanessian反应的脱苄基和同时转化的异头对甲氧基苯基到相应的苯基硫代糖苷在一个步骤中的双重能力。基于此生物素化配体，建立了竞争酶联免疫吸附试验（ELISA），用于检测合成唾液酸苷的结合亲和力。(C)2009爱思唯尔有限公司保留所有权利。
Biotin conjugate of the most potent ligand for mouse CD22 was designed and synthesized. The key synthetic step involved the dual capability of Hanessian reaction for debenzylation and simultaneous conversion of the anomeric p-methoxyphenyl group into the corresponding phenylthioglycoside in one step. Competition enzyme-linked immunosorbent assay (ELISA) for testing the binding affinity of synthetic sialosides was developed based on this biotinylated ligand. (C) 2009 Elsevier Ltd. All rights reserved.