SKIP, the host target of the Salmonella virulence factor SifA, promotes kinesin-1 dependent vacuolar membrane exchanges
SKIP, the host target of the Salmonella virulence factor SifA, promotes kinesin-1 dependent vacuolar membrane exchanges
复制标题
DOI：
10.4161/viru.2.1.14603
复制
发表时间：
2011-01-01
期刊：
影响因子：
5.2
通讯作者：
Meresse, Stephane
中科院分区：
文献类型：
--
作者：
Leone, Philippe;Meresse, Stephane
作为对宿主适应的结果，细胞内病原体已经进化出篡夺和控制真核过程的机制。就沙门氏菌而言，这在一定程度上是通过能够作用于受感染细胞生物学的细菌效应物的细胞质移位实现的。这些细菌效应物可能具有酶活性或靶向真核蛋白。我们已经确定了两个沙门氏菌效应器，它们针对的是正端定向的微管马达Kinesin-1。PipB2是沙门氏菌空泡特异的Kinesin-1货物适配器，而SifA与宿主蛋白Skip结合，后者与微管马达相互作用。Skip是一种功能未知的大型多域蛋白。我们最近的研究表明，Skip以微管和Kinesin-1依赖的方式调节晚期内体隔室的位置。此外，他们指出，在感染和未感染的细胞中，SKIP都激活了微管马达。在这里，我们回顾了这些最新的结果，并提出了一个沙门氏菌效应器介导的Kinesin-1活性调节模型。
As the result of their adaptation to the host, intracellular pathogens have evolved mechanisms to usurp and take the control of eukaryotic processes. In the case of Salmonella, this is in part achieved through the cytoplasmic translocation of bacterial effectors capable of acting on the biology of infected cells. These bacterial effectors might have enzymatic activities or target eukaryotic proteins. We have identified two Salmonella effectors that target the plus- end directed microtubule motor kinesin-1. PipB2 is a Salmonella vacuole-specific cargo adaptor for kinesin-1 while SifA binds the host protein SKIP, which interacts with the microtubule motor. SKIP is a large multi-domain protein of unknown function. Our recent investigations show that SKIP regulates the positioning of late endosomal compartments in a microtubules and kinesin-1 dependent manner. Moreover they indicate that SKIP activates the microtubule motor both in the context of infected and non-infected cells. Here we review these recent results and propose a model for the Salmonella effector-mediated regulation of kinesin-1 activity.