Genetic Deletion ofmiR-430Disrupts Maternal-Zygotic Transition and Embryonic Body Plan
Genetic Deletion ofmiR-430Disrupts Maternal-Zygotic Transition and Embryonic Body Plan
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miR-430 的基因删除破坏母体-合子转变和胚胎体计划
DOI：
10.3389/fgene.2020.00853
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发表时间：
2020-08-04
影响因子：
3.7
通讯作者：
Cheng, Christopher H. K.
中科院分区：
文献类型：
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作者：
Liu, Yun;Zhu, Zeyao;Cheng, Christopher H. K.
MIR-430被认为是胚胎发育过程中的重要调节因子，但遗传功能丧失的研究仍然缺乏。在这里，我们证明了iR-430簇的基因缺失导致了斑马鱼在细胞运动、生殖层规格、轴图案和器官前体形成方面的发育缺陷。转录组分析表明，在iR-430突变体中，母体提供的转录本没有被适当降解，合子基因组表达的基因也没有完全激活。我们进一步发现，miR-430和母亲提供的转录本之间存在一个相互调节的环：母亲提供的转录本(Nanog、Dicer1、Dgcr8和AGOS)是R-430生物发生和功能所必需的，而miR-430是清除这些母亲提供的转录本所必需的。这些数据提供了第一个遗传学证据，表明miR-430是母体向合子转变和随后建立胚胎体计划所必需的。
MiR-430is considered an important regulator during embryonic development, but genetic loss-of-function study is still lacking. Here we demonstrated that genetic deletion of themiR-430cluster resulted in developmental defects in cell movement, germ layer specification, axis patterning and organ progenitor formation in zebrafish. Transcriptome analysis indicated that the maternally provided transcripts were not properly degraded whereas the zygotic genome expressed genes were not fully activated in themiR-430mutants. We further found that a reciprocal regulatory loop exists betweenmiR-430and maternally provided transcripts: the maternally provided transcripts (Nanog,Dicer1,Dgcr8, andAGOs) are required formiR-430biogenesis and function, whereasmiR-430is required for the clearance of these maternally provided transcripts. These data provide the first genetic evidence thatmiR-430is required for maternal-zygotic transition and subsequent establishment of embryonic body plan.