Morphological and Phylogenetic Description of Trypanosoma noyesi sp nov.: An Australian Wildlife Trypanosome within the T-cruzi Clade
Morphological and Phylogenetic Description of Trypanosoma noyesi sp nov.: An Australian Wildlife Trypanosome within the T-cruzi Clade
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DOI：
10.1016/j.protis.2016.07.002
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发表时间：
2016-11-01
期刊：
影响因子：
2.5
通讯作者：
Thompson, R. C. Andrew
中科院分区：
文献类型：
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作者：
Botero, Adriana;Cooper, Crystal;Thompson, R. C. Andrew
来自澳大利亚有袋类动物的许多锥虫分离物都在含有人类病原体克氏锥虫的分支中。这个分支中的锥虫被认为是从共同的祖先蝙蝠锥虫分化而来的。在这里，我们描述了诺伊氏锥虫。11月通过从三个基因区(18S rDNA、gGAPDH和cytb)进行系统发育推断，并结合体外形态和行为观察，从极度濒危的灰斑潜蝇(Bettongia Pencillata)中分离出来。我们还调查了灰头飞狐(Pteropus Polocephalus)的潜在媒介和诺伊斯毛虫的存在。系统发育分析显示，诺伊西毛虫和类似的基因类型集中在克氏毛虫分支的外围。诺伊氏锥虫在形态上与澳大利亚锥虫的其他物种以及克氏锥虫分支内的锥虫都是截然不同的。虽然从感染诺氏锥虫的有袋类动物采集的体外寄生虫和叮咬苍蝇的消化道中没有观察到锥虫，但对诺氏锥虫DNA的检测呈阳性，表明它们是候选媒介。飞狐的组织对诺伊斯毛虫呈阴性反应。这项研究提供了有关克氏锥虫分支中的澳大利亚锥虫的形态和遗传变异性的新信息。(C)2016年提交人(S)。由Elsevier GmbH出版。
A number of trypanosome isolates from Australian marsupials are within the clade containing the human pathogen Trypanosoma cruzi. Trypanosomes within this clade are thought to have diverged from a common ancestral bat trypanosome. Here, we characterise Trypanosoma noyesi sp. nov. isolated from the critically endangered woylie (Bettongia pencillata) using phylogenetic inferences from three gene regions (18S rDNA, gGAPDH, and CytB) coupled with morphological and behavioural observations in vitro. We also investigated potential vectors and the presence of T. noyesi in the grey-headed flying fox (Pteropus poliocephalus). Phylogenetic analysis revealed T. noyesi and similar genotypes grouped at the periphery of the T. cruzi clade. T. noyesi is morphologically distinct both from other species of Australian trypanosomes and those within the T. cruzi clade. Although trypanosomes were not observed in the digestive tract of ectoparasites and biting flies collected from T. noyesi infected marsupials, tabanid and biting midges tested positive for T. noyesi DNA, indicating they are vector candidates. Tissues from flying foxes were negative for T. noyesi. This study provides novel information on the morphology and genetic variability of an Australian trypanosome within the T. cruzi clade. (C) 2016 The Author(s). Published by Elsevier GmbH.