A microscope-based screening platform for large-scale functional protein analysis in intact cells
A microscope-based screening platform for large-scale functional protein analysis in intact cells
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DOI：
10.1016/s0014-5793(03)01197-9
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发表时间：
2003-11-20
期刊：
影响因子：
3.5
通讯作者：
Pepperkok, R
中科院分区：
文献类型：
--
作者：
Liebel, U;Starkuviene, V;Pepperkok, R
描述了一种基于模块化显微镜的筛选平台，用于完整细胞中蛋白质功能的大规模分析。它包括自动样品制备，图像采集，数据管理和分析，以及全基因组生物信息学信息的自动检索。该系统的模块化特性确保了它能够快速适应新的生物学问题或蛋白质组。开发并测试了两种解决蛋白质分泌和高尔基复合体完整性的自动功能分析。这显示了该系统在大规模、基于细胞的功能蛋白质组学项目中的潜力。(C) 2003年欧洲生化学会联合会。Elsevier B.V.版权所有。
A modular microscope-based screening platform, with applications in large-scale analysis of protein function in intact cells is described. It includes automated sample preparation, image acquisition, data management and analysis, and the genome-wide automated retrieval of bioinformatic information. The modular nature of the system ensures that it is rapidly adaptable to new biological questions or sets of proteins. Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.