Plant regeneration from immature seed-derived callus of Cypripedium macranthos swartz var. taiwanianum (Masamune) F. Maekawa
Plant regeneration from immature seed-derived callus of Cypripedium macranthos swartz var. taiwanianum (Masamune) F. Maekawa
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未成熟种子来源的愈伤组织再生植物
DOI：
10.1270/jsbbs1951.47.279
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Mina Tomita
中科院分区：
文献类型：
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作者：
M. Tomita;Mina Tomita
为给濒危植物Cypripediummacranthasvar. taiwanianum进行了研究。将在补充有200 mg/l酵母提取物、10 g/l蔗糖和2 g/l结冷胶的T培养基（Tsutsui和Tomita 1990）上由未成熟种子诱导的愈伤组织用于植物再生试验。在含有10 ~（-5）M NAA和10 ~（-6）M BAP的培养基上，观察到三个愈伤组织系的再生。春化处理后，建立再生植株。
To obtain basic information for the micropropagation and future breeding of endangered Cypripedium species, plant regeneration from immature seed-derived callus of Cypripedium macranthas var. taiwanianum was studied. Calli, induced from immature seeds on T medium (Tsutsui and Tomita 1990) supplernented with 200 mg/l yeast extract, 10 g/l sucrose and 2 g/l gellan gum, were used for the plant regeneration test. The regeneration was observed in three lines of callus on media containing 10-5M NAA with or without the addition of 10-6M BAP. After vernalization, regenerated plants were established.