Formation of the notochord in living ascidian embryos.
Formation of the notochord in living ascidian embryos.
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发表时间：
1985-04
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影响因子：
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通讯作者：
D. M. Miyamoto;R. J. Crowther
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作者：
D. M. Miyamoto;R. J. Crowther
用微分干涉对比显微镜和延时录像技术研究了海鞘（Ciona dendritis）脊索形成过程中细胞的动态行为。原始原基的形成部分是由于有丝分裂的模式，随着胚孔向后移动。垂直和水平重排产生端对端堆叠的盘形细胞的细长棒。进一步的伸长伴随着细胞形状的变化。一些细胞生长或肿胀表明发生在发育后期，但这种生长似乎主要是对直径的增加，而对脊索长度的增加并不显着。受精后13 h左右出现的胞内空泡体积增大，16 h左右融合形成胞间空泡。在18 ~ 20 h左右，这些细胞又合并形成脊索的中央基质核心。随着脊索的伸长和细胞形状的改变，基底面的水泡活跃。这种表面活性可能与围索鞘的形成有关。
The dynamic behaviour of cells during formation of the notochord in the ascidian, Ciona intestinalis, was examined by means of Differential Interference Contrast (DIC) microscopy and time-lapse videorecording. The initial rudiment is formed in part as a consequence of the pattern of mitotic divisions as the blastopore shifts posteriorly. Vertical and horizontal rearrangements produce an elongate rod of disc-shaped cells stacked end to end. Further elongation is accompanied by a cell shape change. Some cell growth or swelling is indicated to occur later in development, but this growth appears to contribute mostly to an increase in the diameter, and only insignificantly to the length of the notochord. Intracellular vacuoles that appear around 13 h after fertilization increase in size and fuse at about 16 h form intercellular ones. These in turn merge to form the central matrix core of the notochord at around 18 to 20 h. As the notochord elongates and cells change in shape, the basal surfaces bleb actively. This surface activity may be related to formation of the perinotochordal sheath.