Epidemiological Survey of Theileria orientalis Infection in Grazing Cattle in the Eastern Part of Hokkaido, Japan
Epidemiological Survey of Theileria orientalis Infection in Grazing Cattle in the Eastern Part of Hokkaido, Japan
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DOI：
10.1292/jvms.71.937
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发表时间：
2009-07-01
影响因子：
1.2
通讯作者：
Yokoyama, Naoaki
中科院分区：
文献类型：
--
作者：
Ota, Naomi;Mizuno, Daisuke;Yokoyama, Naoaki
东方喜勒菌（Theileria orientalis）是日本广泛分布的喜勒菌属（Theileria orientalis）中的一种，有时会给畜牧业造成严重的经济损失。本研究以北海道东部地区（Taiki、Otofuke、Shintoku和shinhidaka地区）放牧牛为研究对象，结合传统诊断方法和分子检测方法，调查了牛东方绦虫感染现状。采用高检出率的聚合酶链反应（PCR）技术，鉴定了东方桃主要梨质表面蛋白（MPSP）和p23的编码基因。MPSP-PCR检测结果表明，放牧1.5个月后，这些地区的放牧牛感染率较高，为10.0 ~ 64.8%。虽然检测到的MPSP和p23基因型主要为池田型或壳聚糖型，但在新德和新飞隆地区的牛中发现了与冲绳县密切相关的MPSP基因和与澳大利亚（Warwick） buffeli型基因密切相关的p23基因。本调查结果显示，日本北海道地区至少有5种按MPSP基因分类的东方T虫，且该地区东方T虫感染率仍然较高。
Theileria orientalis is one of the benign species of Theileria that is widely distributed in Japan and is sometimes responsible for serious economic losses in the livestock industry. tit the present Study, we surveyed the current status of T. orientalis infection in grazing cattle in the eastern areas of Hokkaido (Taiki, Otofuke, Shintoku, and Shin-Hidaka districts) using molecular methods, as well as traditional methods, of diagnosis. The genes encoding the major piroplasm surface protein (MPSP) and p23 of T orientalis were identified using highly detectable polymerase chain reaction (PCR). Results of the MPSP-PCR assay indicated that grazing cattle in these districts, after about 1.5 months pasturage, showed high rates of infection, ranging from 10.0-64.8%. Although the main MPSP and p23 genotypes detected were the Ikeda- or Chitose-types, an MPSP gene closely relating to that Found in Okinawa prefecture, and a p23 gene closely relating to the Australian (Warwick) Buffeli-type gene, were found in the cattle in Shintoku and Shin-Hidaka districts. The present Survey indicated that there were at least Five types of T orientalis classified by their MPSP genes in Hokkaido, Japan, and that T orientalis infection rates are still high in this region.