Mycorrhizal synthesis, morpho-anatomical characterization of mycorrhizae, and evaluation of mycorrhiza-forming ability of Hydnum albidum-like species using monokaryotic and dikaryotic cultures.
Mycorrhizal synthesis, morpho-anatomical characterization of mycorrhizae, and evaluation of mycorrhiza-forming ability of Hydnum albidum-like species using monokaryotic and dikaryotic cultures.
复制标题
使用单核和双核培养物进行菌根合成、菌根形态解剖学表征以及类似 Hydnum albidum 物种的菌根形成能力评估。
DOI：
10.1007/s00572-021-01024-7
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发表时间：
2021
期刊：
影响因子：
3.9
通讯作者：
Endo N.
中科院分区：
文献类型：
--
作者：
Sugawara R;Sotome K;Maekawa N;Nakagiri A;Endo N.
尽管Hydnum物种的经济和生态的重要性，在体外合成的外生真菌的本属还没有报道，由于在建立纯培养的困难。将12个单核和3个双孢菌丝体菌株的纯培养物接种于无菌的赤松幼苗根部，以合成外生菌根并评价其形成菌根的能力。接种后6个月，单核和双核菌株都在根皮层形成了具有哈蒂格网菌丝的外生菌根。单核和双孢菌株表现出相似的形态解剖学特征的外生真菌，除了夹紧隔膜的发射和外地幔菌丝在后者。单核和后代菌株之间，有没有显着差异，在松苗的菌根真菌的数量，而单核菌株表现出更大的根尖总数和较低的定殖率比后代菌株。这些结果表明，无论是单核菌丝还是双核菌丝，H.类albidum-like种在无菌条件下可形成真菌，可用于栽培刺菇。
Despite the economic and ecological importance ofHydnumspecies, in vitro synthesis of ectomycorrhizae of this genus has not been reported due to difficulties in establishing pure cultures. We inoculated pure cultures of 12 monokaryotic and 3 dikaryotic mycelial strains of an undescribedHydnum albidum-like species on roots of axenicPinus densifloraseedlings to synthesize ectomycorrhizae and to evaluate their mycorrhiza-forming ability. Six months after inoculation, both monokaryotic and dikaryotic strains formed ectomycorrhizae with Hartig net hyphae at the root cortex. Monokaryotic and dikaryotic strains exhibited similar morpho-anatomical characteristics of ectomycorrhizae, with the exception for clamped septa of emanating and outer mantle hyphae in the latter. Between monokaryotic and descendant dikaryotic strains, there were no significant differences in number of mycorrhizae in pine seedlings, whereas monokaryotic strains showed a greater total number of root tips and lower colonization rates than the descendant dikaryotic strains. These results indicate that both monokaryotic and dikaryotic mycelia of theH. albidum-like species can form mycorrhizae under axenic condition, and that can be applied toward the cultivation of hedgehog mushrooms.