Tailored Electroactive and Quantitative Ligand Density Microarrays Applied to Stem Cell Differentiation
Tailored Electroactive and Quantitative Ligand Density Microarrays Applied to Stem Cell Differentiation
复制标题
DOI：
10.1021/ja907187f
复制
发表时间：
2010-03-03
影响因子：
15
通讯作者：
Yousaf, Muhammad N.
中科院分区：
文献类型：
--
作者：
Luo, Wei;Chan, Eugene W. L.;Yousaf, Muhammad N.
在分子水平上精确控制材料和哺乳动物细胞之间相互作用的能力对于理解局部环境如何影响细胞行为的基本化学性质以及开发用于一系列生物技术和组织工程应用的新生物材料至关重要。在这份报告中，我们开发和应用的第一次定量电活性微阵列策略，可以提出各种配体与精确控制配体密度研究人骨髓间充质干细胞（hMSC）分化透明表面上的一种新的方法来定量成脂分化。我们发现配体组成和配体密度都影响hMSC的成脂分化率。此外，这种新的分析生物技术方法与其他生物界面表征技术（表面等离子体共振，质谱法）兼容，也可用于研究各种系统生物学研究或基于细胞行为的测定的蛋白质-配体或细胞-材料相互作用。
The ability to precisely control the interactions between materials and mammalian cells at the molecular level is crucial to understanding the fundamental chemical nature of how the local environment influences cellular behavior as well as for developing new biomaterials for a range of biotechnological and tissue engineering applications. In this report, we develop and apply for the first time a quantitative electroactive microarray strategy that can present a variety of ligands with precise control over ligand density to study human mesenchymal stem cell (hMSC) differentiation on transparent surfaces with a new method to quantitate adipogenic differentiation. We found that both the ligand composition and ligand density influence the rate of adipogenic differentiation from hMSC's. Furthermore, this new analytical biotechnology method is compatible with other biointerfacial characterization technologies (surface plasmon resonance, mass spectrometry) and can also be applied to investigate a range of protein-ligand or cell-material interactions for a variety of systems biology studies or cell behavior based assays.