Small introns in a hepatic cDNA encoding a new glucagon-like peptide 1-type receptor.
Small introns in a hepatic cDNA encoding a new glucagon-like peptide 1-type receptor.
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肝脏 cDNA 中的小内含子编码新的胰高血糖素样肽 1 型受体。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
Jean Christophe
中科院分区：
文献类型：
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作者：
M. Svoboda;E. Ciccarelli;M. Tastenoy;A. Cauvin;M. Stievenart;Jean Christophe
利用与分泌素受体家族成员的第二和第三跨膜段的一致序列相对应的两个退化引物，从大鼠DNA中以低强度PCR扩增出cDNA片段。基于该片段3端的合成寡脱氧核苷酸，当用于Northern-blot分析时，在大鼠肝脏中杂交出1.5-3.0 kb的宽条带。然后将其用作探针，筛选由大小分离的大鼠肝脏poly(a)+ RNA生成的cDNA文库。对3个克隆的序列进行重叠比对，鉴定出一个1455个残基的开放阅读框，编码485个氨基酸的肽，显示7个推测的跨膜片段，42%与胰高血糖素样肽1 （GLP-1）受体同源。此外，4个小内含子（85-300 bp）在分离的克隆中有不同的表现。
A cDNA fragment was PCR amplified at low-stringency from rat DNA, using two degenerated primers corresponding to consensus sequences in the second and third transmembrane segments of members of the secretin receptor family. A synthetic oligodeoxynucleotide based on the 3 end of this fragment, when used in Northern-blot analysis, hybridized to a broad 1.5-3.0 kb band in rat liver. It was then used as a probe for screening a cDNA library generated from size-fractionated rat liver poly(A)+ RNA. Overlapping alignment of sequences from 3 clones identified a 1455 residue open reading frame encoding a 485 amino acid peptide showing 7 putative transmembrane segments and 42% identity with the glucagon-like peptide 1 (GLP-1) receptor. In addition, four small (85-300 bp) introns were variously represented in the isolated clones.