A non-canonical function of Arabidopsis ERECTA proteins and a role of the SWI3B subunit of the SWI/SNF chromatin remodeling complex in gibberellin signaling.
A non-canonical function of Arabidopsis ERECTA proteins and a role of the SWI3B subunit of the SWI/SNF chromatin remodeling complex in gibberellin signaling.
复制标题
拟南芥 ERECTA 蛋白的非典型功能以及 SWI/SNF 染色质重塑复合物的 SWI3B 亚基在赤霉素信号传导中的作用。
DOI：
10.1111/tpj.16261
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Sarnowska E
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文献类型：
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作者：
Sarnowska E
拟南芥 ERECTA 家族 (ERf) 富含亮氨酸重复受体样激酶 (LRR-RLK)，包括 ERECTA (ER)、ERECTA-LIKE 1 (ERL1) 和 ERECTA-LIKE 2 (ERL2)，控制表皮图案、花序结构以及气孔发育和图案。据报道这些蛋白质与质膜相关。在这里，我们发现 theer/erl1/erl2 突变体表现出赤霉素 (GA) 生物合成和感知受损以及广泛的转录变化。研究发现 ERf 激酶结构域定位于细胞核，与 SWI/SNF 染色质重塑复合体 (CRC) 的 SWI3B 亚基相互作用。 Theer/erl1/erl2 突变体表现出 SWI3B 蛋白水平降低并影响核小体染色质结构。与具有失活的 SWI/SNF CRC 亚基的 swi3candbr 植入物类似，它也不会积累 DELLA RGA 和 GAI 蛋白。 ER激酶在体外磷酸化SWI3B，所有ERf蛋白的失活导致体内SWI3B蛋白磷酸化降低。 DELLA 过度积累与 SWI3B 蛋白酶体降解之间的相关性以及 SWI3B 与 DELLA 蛋白的物理相互作用表明含有 SWI3B 的 SWI/SNF CRC 在赤霉素信号传导中发挥重要作用。 ER 和 SWI3B 在 GID1（GIBBERELLIN INSENSITIVE DWARF 1）DELLA 靶基因启动子区域的共定位以及在 er/erl1/erl2 植物中废除的 SWI3B 与 GID1 启动子的结合支持了以下结论：ERf-SWI/SNF CRC 相互作用对于 GA 受体的转录控制很重要。因此，ERf 蛋白参与基因表达的转录控制，并观察到人类 HER2（表皮生长家族受体成员）的相似特征，为进一步研究真核细胞膜受体的进化保守的非规范功能提供了一个令人兴奋的目标。
TheArabidopsisERECTA family (ERf) of leucine‐rich repeat receptor‐like kinases (LRR‐RLKs) comprising ERECTA (ER), ERECTA‐LIKE 1 (ERL1), and ERECTA‐LIKE 2 (ERL2) controls epidermal patterning, inflorescence architecture, and stomata development and patterning. These proteins are reported to be plasma membrane associated. Here we show that theer/erl1/erl2mutant exhibits impaired gibberellin (GA) biosynthesis and perception alongside broad transcriptional changes. The ERf kinase domains were found to localize to the nucleus where they interact with the SWI3B subunit of the SWI/SNF chromatin remodeling complex (CRCs). Theer/erl1/erl2mutant exhibits reduced SWI3B protein level and affected nucleosomal chromatin structure. Similar toswi3candbrmplants with inactivated subunits of SWI/SNF CRCs, it also does not accumulate DELLA RGA and GAI proteins. The ER kinase phosphorylates SWI3Bin vitro, and the inactivation of all ERf proteins leads to the decreased phosphorylation of SWI3B proteinin vivo. The identified correlation between DELLA overaccumulation and SWI3B proteasomal degradation, and the physical interaction of SWI3B with DELLA proteins indicate an important role of SWI3B‐containing SWI/SNF CRCs in gibberellin signaling. Co‐localization of ER and SWI3B onGID1(GIBBERELLIN INSENSITIVE DWARF 1) DELLA target gene promoter regions and abolished SWI3B binding to GID1 promoters in er/erl1/erl2 plants supports the conclusion that ERf‐SWI/SNF CRC interaction is important for transcriptional control of GA receptors. Thus, the involvement of ERf proteins in the transcriptional control of gene expression, and observed similar features for human HER2 (epidermal growth family receptor member), indicate an exciting target for further studies of evolutionarily conserved non‐canonical functions of eukaryotic membrane receptors.