Experimental aspects of biological X-ray absorption spectroscopy
Experimental aspects of biological X-ray absorption spectroscopy
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DOI：
10.1107/s0909049502022598
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发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
Murphy, L
中科院分区：
文献类型：
--
作者：
Ascone, I;Meyer-Klaucke, W;Murphy, L
光谱技术，如X射线吸收光谱学，将为基因组学和蛋白质组学的综合生物学项目提供重要的投入。本投稿总结了简单和复杂生物XAS实验的技术要求和典型设置。对不同的样品制备策略进行了详细的综述。介绍了目前和未来的BioXAS光谱仪，以帮助潜在用户定位其生物应用所需的光谱仪。
Spectroscopic techniques, like X-ray absorption spectroscopy, will provide important input for integrated biological projects in genomics and proteomics. This contribution summarizes technical requirements and typical set-ups for both simple and complex biological XAS experiments. An overview on different strategies for sample preparation is discussed in detail. Present and future BioXAS spectrometers are presented to help potential users in locating the spectrometer required for their biological application.