Sexing chick embryos: a rapid and simple protocol
Sexing chick embryos: a rapid and simple protocol
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DOI：
10.1080/713655025
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发表时间：
2001-03-01
影响因子：
2
通讯作者：
McBride, D
中科院分区：
文献类型：
--
作者：
Clinton, M;Haines, L;McBride, D
1. 分析发育中的鸡性腺中的基因表达需要从发育 3.5 天到 8.5 天的胚胎中收集雄性和雌性组织。然而，在发育第7.5天之前，通过形态学检查无法区分雄性和雌性鸡胚胎。2．早期胚胎的性别鉴定只能通过分子方法实现，目前这种方法既费力又费时。3．我们设计了一种基于 PCR 的性别鉴定方案，该方案将性别特异性反应和对照反应结合在单管测定中。该测定在较宽的 DNA 浓度范围内快速有效，并且可以容忍劣质 DNA。4。描述了使用组织样本或从羊水中回收的少量细胞来识别单个胚胎性别的程序。
1. Analysis of gene expression in the developing chick gonads requires the collection of male and female tissues from embryos between 3.5 d and 8.5 d of development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development.2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming.3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA.4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid.