THE EFFECT OF METHYLATION INHIBITORS ON CITREAMICIN BIOSYNTHESIS IN MICROMONOSPORA-CITREA
THE EFFECT OF METHYLATION INHIBITORS ON CITREAMICIN BIOSYNTHESIS IN MICROMONOSPORA-CITREA
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DOI：
10.7164/antibiotics.44.1247
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发表时间：
1991-11-01
影响因子：
3.3
通讯作者：
MAIESE, WM
中科院分区：
文献类型：
--
作者：
PEARCE, CJ;CARTER, GT;MAIESE, WM
当在甲基化抑制剂sinefungin或氨基蝶呤的存在下孵育产生柠檬酸霉素的生物体Micromonospora citrea NRRL 189351时，zeta组分的生物合成被刺激为比正常产生的水平高约20至200倍。 没有检测到对第二个甲基化反应的抑制，第二个甲基化反应表面上与第一个非常相似。 其他已知的甲基化抑制剂未能产生所产生的桔霉素的天然模式的任何变化。 这种方法是一个很好的路线，用于制备citreamicin-zeta，它可以用作半合成或进一步的生物合成研究的底物。
When the citreamicin-producing organism Micromonospora citrea NRRL 189351 was incubated in the presence of the methylation inhibitors sinefungin or aminopterin, biosynthesis of the zeta component was stimulated approximately 20 to 200-fold above the level normally produced. Inhibition of a second methylation reaction, which is superficially very similar to the first, was not detected. Other known methylation inhibitors failed to yield any change in the natural pattern of citreamicins produced. This approach is an excellent route for preparing citreamicin-zeta, which can be used as a substrate for semi-synthesis or for further biosynthetic studies.