Involvement of adenosine A1 receptor in electroacupuncture-mediated inhibition of astrocyte activation during neuropathic pain
Involvement of adenosine A1 receptor in electroacupuncture-mediated inhibition of astrocyte activation during neuropathic pain
复制标题
腺苷 A1 受体参与电针介导的神经病理性疼痛期间星形胶质细胞激活的抑制
DOI：
10.1590/0004-282x20180128
复制
发表时间：
2018-11-01
影响因子：
1.4
通讯作者：
Wang, Junlu
中科院分区：
文献类型：
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作者：
Zhang, Mingxiao;Dai, Qinxue;Wang, Junlu
神经性疼痛是一种由中枢或周围神经系统损伤或功能障碍引起的慢性疼痛状况。电针（EA）对神经性疼痛具有抗痛觉作用，其部分原因是抑制脊髓星形胶质细胞的激活。我们发现鞘内注射8-环戊基-1,3-二丙基黄嘌呤（DPCPX），一种选择性腺苷A1受体拮抗剂，逆转了EA在慢性收缩性损伤诱导的神经性疼痛模型中的抗伤害性作用。GFAP在L4-L6脊髓中的表达显著升高，而DPCPX抑制了ea介导的星形胶质细胞活化的抑制作用，并消除了ea诱导的细胞因子含量（TNF-α）的抑制作用。这些结果提示腺苷A1受体通过抑制EA的星形细胞活化和TNF-α上调，参与了神经性疼痛过程中EA的作用，为针刺镇痛的机制和神经性疼痛治疗靶点的开发提供了启示。
ABSTRACT Neuropathic pain is a chronic pain condition caused by damage or dysfunction of the central or peripheral nervous system. Electroacupuncture (EA) has an antinociceptive effect on neuropathic pain, which is partially due to inhibiting astrocyte activation in the spinal cord. We found that an intrathecal injection of 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), a selective adenosine A1 receptor antagonist, reversed the antinociceptive effects of EA in a chronic constriction injury-induced neuropathic pain model. The expression of GFAP in L4-L6 spinal cord was significantly upgraded, while DPCPX suppressed the effect of the EA-mediating inhibition of astrocyte activation, as well as wiping out the EA-induced suppression of cytokine content (TNF-α). These results indicated that the adenosine A1 receptor is involved in EA actions during neuropathic pain through suppressing astrocyte activation as well as TNF-α upregulation of EA, giving enlightenment to the mechanisms of acupuncture analgesia and development of therapeutic targets for neuropathic pain.