Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence.
Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence.
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DOI：
10.1016/j.fertnstert.2012.04.035
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发表时间：
2012-08-01
影响因子：
6.7
通讯作者：
Woodruff, Teresa K
中科院分区：
文献类型：
--
作者：
Jiao, Ze-Xu;Xu, Min;Woodruff, Teresa K
目的：为了证实在中期II（MII）卵母细胞的极体（PB）中可检测到卵母细胞特异性信使RNA，并确定年龄对卵母细胞特异性转录水平的影响。用孕马血清促性腺激素（PMSG）和hCG分别对40-50周龄和7-9周龄的小鼠进行了实验研究。卵母细胞体外受精，以评估受精和发育能力。获得MII卵母细胞并取出第一批PB。采用实时荧光定量PCR（real-time quantitative polymerase chain reaction，PCR）技术对每个PB及其同胞卵母细胞的mRNA进行逆转录和分析。主要观察指标：受精率、发育率和6个卵母细胞特异性基因的表达（Bmp 15、Gdf 9、H1 foo、Nlrp 5、Tcl 1和Zp 3）在来自年轻小鼠与老年小鼠的PB和同胞卵母细胞中的表达。老年小鼠卵母细胞发育能力较低。四个基因（H1 foo，Nlrp 5，Tcl 1和Zp 3）在老年与年轻卵母细胞中差异表达。所有6个转录本存在于PBs从老年和年轻的小鼠在较低的水平比在兄弟姐妹的卵母细胞;转录水平较低，在老年PBs与年轻PBs.CONCLUSION（S）：有一个显着的差异，在卵母细胞特异性基因的转录水平与年龄相关的卵母细胞的能力下降，在老年与年轻的PB。PB中基因表达的差异可能是MII卵母细胞能力的潜在生物标志物。
OBJECTIVE: To confirm that oocyte-specific messenger RNAs are detectable in the polar body (PB) of metaphase II (MII) oocytes and determine the effect of age on oocyte-specific transcript levels.DESIGN: Prospective study.SETTING: Hospital-based academic research laboratory.ANIMAL(S): CD1 female mice.INTERVENTION(S): Aged (40-50 weeks) and young (7-9 weeks) mice were administered pregnant mare serum gonadotropin (PMSG) and hCG. Oocytes were fertilized in vitro to assess fertilization and developmental competence. The MII oocytes were obtained and first PBs were removed. Messenger RNAs from each PB and its sibling oocyte were reverse transcribed and analyzed by real-time quantitative polymerase chain reaction (PCR).MAIN OUTCOME MEASURE(S): Fertilization and developmental rates and expression of six oocyte-specific genes (Bmp15, Gdf9, H1foo, Nlrp5, Tcl1, and Zp3) in PBs and sibling oocytes from young versus aged mice.RESULT(S): Oocytes from aged mice had lower developmental competence. Four genes (H1foo, Nlrp5, Tcl1, and Zp3) were differentially expressed in aged versus young oocytes. All six transcripts were present in PBs from aged and young mice at lower levels than in the sibling oocytes; transcript levels were lower in aged PBs compared with young PBs.CONCLUSION(S): There is a significant difference in the transcript levels of oocyte-specific genes in aged versus young PB that correlates with age-related decreases in oocyte competence. Differences in gene expression in PB may be potential biomarkers of MII oocyte competence.