Arabidopsis TERMINAL FLOWER1 Is Involved in the Regulation of Flowering Time and Inflorescence Development through Transcriptional Repression
Arabidopsis TERMINAL FLOWER1 Is Involved in the Regulation of Flowering Time and Inflorescence Development through Transcriptional Repression
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DOI：
10.1105/tpc.111.088641
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发表时间：
2011-09-01
期刊：
影响因子：
11.6
通讯作者：
Goto, Koji
中科院分区：
文献类型：
--
作者：
Hanano, Shigeru;Goto, Koji
TFL 1是拟南芥开花时间和花序分生组织发育的关键调控因子。TFL 1和FLOW LOCUS T（FT）具有高度保守的氨基酸序列，但功能相反。例如，FT促进开花和TFL 1抑制它; FT过表达的植物和TFL 1功能丧失突变体具有类似的表型生产的顶生花在茎尖。FT被认为通过与FD相互作用在转录激活因子复合物中起作用。在这里，我们证明了TFL 1参与了FT激活的基因的转录抑制。我们分析了过表达TFL 1融合到转录抑制结构域（TFL 1-SRDX）或激活结构域（TFL 1-VP 16）的转基因植物。携带35 S：TFL 1-SRDX的植物表现出与35 S：TFL 1植物相似的延迟开花，携带35 S：TFL 1-VP 16的植物表现出早花表型并产生顶生花。此外，TFL 1和35 S：TFL 1-VP 16植物表型强烈抑制的fd突变，和TFL 1与FD在细胞核中，如双分子荧光互补实验所示。我们的结论是TFL 1负调节FD依赖的转录靶基因微调开花时间和花序分生组织的发展。
TERMINAL FLOWER1 (TFL1) is a key regulator of flowering time and the development of the inflorescence meristem in Arabidopsis thaliana. TFL1 and FLOWERING LOCUS T (FT) have highly conserved amino acid sequences but opposite functions. For example, FT promotes flowering and TFL1 represses it; FT-overexpressing plants and TFL1 loss-of-function mutants have a similar phenotype production of terminal flowers in the shoot apex. FT is believed to function in a transcriptional activator complex by interacting with FD. Here, we demonstrate that TFL1 is involved in the transcriptional repression of genes that are activated by FT. We analyzed transgenic plants overexpressing TFL1 fused to a transcriptional repressor domain (TFL1-SRDX) or an activator domain (TFL1-VP16). Plants carrying 35S:TFL1-SRDX showed delayed flowering similar to 35S: TFL1 plants, and plants carrying 35S: TFL1-VP16 showed an early flowering phenotype and produced terminal flowers. Furthermore, the tfl1 and 35S: TFL1-VP16 plant phenotypes were strongly suppressed by the fd mutation, and TFL1 interacted with FD in the cell nucleus, as shown by bimolecular fluorescence complementation experiments. We conclude that TFL1 negatively modulates the FD-dependent transcription of target genes to fine-tune flowering time and the development of the inflorescence meristem.