Determination of Ag[I] and NADH Using Single-Molecule Conductance Ratiometric Probes
Determination of Ag[I] and NADH Using Single-Molecule Conductance Ratiometric Probes
复制标题
使用单分子电导比率探针测定 Ag[I] 和 NADH
DOI：
10.1021/acssensors.0c02038
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发表时间：
2021-02-26
期刊：
影响因子：
8.9
通讯作者：
Hong, Wenjing
中科院分区：
文献类型：
--
作者：
Hu, Yong;Zhuang, Xiaoyan;Hong, Wenjing
基于单分子测量的传感平台为构建超灵敏系统提供了新的视角。然而，大多数这些传感平台无法准确测定目标分析物。在此，我们展示了电导比率策略与单分子电导技术相结合，以实现超灵敏和精确的测定。以3,3',5,5'-四甲基联苯胺(TMB)和氧化TMB(oxTMB)为电导比率探针构建单分子传感平台,应用于Ag[I]和烟酰胺腺嘌呤二核苷酸(NADH)的检测。结果发现，TMB 和 oxTMB 的电荷传输特性截然不同，电导变化超过一个数量级，因此可以对实际样品中的 Ag[I] 和 NADH 进行电导比率分析。该方法具有超灵敏性，并且在复杂矩阵中具有抗干扰能力。检测限可低至阿摩尔浓度（类似于 34 aM）。我们相信，所提出的电导比率方法通常足以具有广泛而复杂的分析的良好潜力。
The sensing platform based on single-molecule measurements provides a new perspective for constructing ultrasensitive systems. However, most of these sensing platforms are unavailable for the accurate determination of target analytes. Herein, we demonstrate a conductance ratiometric strategy combing with the single-molecule conductance techniques for ultrasensitive and precise determination. A single-molecule sensing platform was constructed with the 3,3',5,5'-tetramethylbenzidine (TMB) and oxidized TMB (oxTMB) as the conductance ratiometric probes, which was applied in the detection of Ag[I] and nicotinamide adenine dinucleotide (NADH). It was found that the charge transport properties of TMB and oxTMB were distinct with more than an order of magnitude change of the conductance, thus enabling conductance ratiometric analysis of the Ag[I] and NADH in the real samples. The proposed method is ultrasensitive and has an anti-interference ability in the complicated matrix. The limit of detection can be as low as attomolar concentrations (similar to 34 aM). We believe that the proposed conductance ratiometric approach is generally enough to have a promising potential for broad and complicated analysis.