Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling
Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling
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DOI：
10.1091/mbc.e07-01-0071
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发表时间：
2007-06-01
影响因子：
3.3
通讯作者：
Fujimoto, Toyoshi
中科院分区：
文献类型：
--
作者：
Fujita, Akikazu;Cheng, Jinglei;Fujimoto, Toyoshi
已经假定在细胞膜中存在微区，但是脂质分子的二维分布难以在亚微米尺度上确定。在本文中，我们研究了神经节苷脂GM1和GM3，假定筏分子在细胞膜上的分布，免疫电镜使用速冻和冷冻断裂标本。该方法将分子物理固定在原位，从而使人为扰动的可能性最小化。通过免疫金标记的点模式分析，显示GM 1使
Presence of microdomains has been postulated in the cell membrane, but two-dimensional distribution of lipid molecules has been difficult to determine in the submicrometer scale. In the present paper, we examined the distribution of gangliosides GM1 and GM3, putative raft molecules in the cell membrane, by, immunoelectron microscopy using quick-frozen and freeze-fractured specimens. This method physically immobilized molecules in situ and thus minimized the possibility of artifactual perturbation. By point pattern analysis of immunogold labeling, GM1 was shown to make clusters of