Bone marrow mesenchymal stem cells decrease CHOP expression and neuronal apoptosis after spinal cord injury
Bone marrow mesenchymal stem cells decrease CHOP expression and neuronal apoptosis after spinal cord injury
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骨髓间充质干细胞减少脊髓损伤后 CHOP 表达和神经元凋亡
DOI：
10.1016/j.neulet.2016.11.032
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发表时间：
2017-01-01
影响因子：
2.5
通讯作者：
Wang, Linlin
中科院分区：
文献类型：
--
作者：
Gu, Chuanlong;Li, Heyangzi;Wang, Linlin
脊髓损伤（SCI）可导致不可逆的神经元丧失，最终导致瘫痪。骨髓间充质干细胞（BMSCs）已被证明是治疗脊髓损伤的有效方法。本研究旨在探讨骨髓间充质干细胞在脊髓损伤大鼠和氧葡萄糖剥夺（OGD）处理的运动神经元中的作用。结果表明，骨髓间质干细胞能改善脊髓损伤后大鼠的运动功能，降低促凋亡转录因子C/EBP同源蛋白（pro- apoptosis transcription factor C/EBP homologous protein， CHOP）的表达和细胞凋亡。此外，与骨髓间充质干细胞或骨髓间充质干细胞的条件培养基共培养也可降低ogd后运动神经元CHOP的表达和凋亡，支持骨髓间充质干细胞通过降低损伤运动神经元CHOP的表达发挥保护作用。我们的发现为骨髓间充质干细胞治疗脊髓损伤患者提供了一种潜在的新机制。(C) 2016由爱思唯尔爱尔兰有限公司出版。
Spinal cord injury (SCI) leads to irreversible neuronal loss and ultimately leads to paralysis. Bone marrow derived mesenchymal stem cells (BMSCs) have been demonstrated to be an effective approach to treat SCI. The present study was designed to investigate the role of BMSCs in rats with spinal cord injury and in oxygen-glucose deprivation (OGD) treated motor neurons. The results demonstrated that BMSCs could improve locomotor function and decrease expression of pro-apoptotic transcription factor C/EBP homologous protein (CHOP) and apoptosis after SCI. Furthermore, co-culture with BMSCs or conditioned medium from BMSCs could also decrease the expression of CHOP and apoptosis in post-OGD motor neurons, supporting that BMSCs exerts protective effects by decreasing the expression of CHOP in injured motor neurons. Our findings provide a potential novel mechanism for BMSCs treatments in patients with SCI. (C) 2016 Published by Elsevier Ireland Ltd.