Role of Intracellular Proteases in the Antibiotic Resistance, Motility, and Biofilm Formation of Pseudomonas aeruginosa
Role of Intracellular Proteases in the Antibiotic Resistance, Motility, and Biofilm Formation of Pseudomonas aeruginosa
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DOI：
10.1128/aac.05336-11
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发表时间：
2012-02-01
影响因子：
4.9
通讯作者：
Hancock, Robert E. W.
中科院分区：
文献类型：
--
作者：
Fernandez, Lucia;Breidenstein, Elena B. M.;Hancock, Robert E. W.
铜绿假单胞菌具有复杂的调控网络，控制在不利条件下的毒力和存活，包括抗生素压力，它们相互关联并共享共同的调控蛋白。在这里，我们筛选了一组13个细胞内蛋白酶缺陷突变体，并证明，除了已知的Lon和AsrA突变体的改变，三个蛋白酶相关蛋白PfPI，ClpS和ClpP的突变差异影响抗生素耐药性，群集运动，和生物膜形成。
Pseudomonas aeruginosa possesses complex regulatory networks controlling virulence and survival under adverse conditions, including antibiotic pressure, which are interconnected and share common regulatory proteins. Here, we screen a panel of 13 mutants defective in intracellular proteases and demonstrate that, in addition to the known alterations in Lon and AsrA mutants, mutation of three protease-related proteins PfpI, ClpS, and ClpP differentially affected antibiotic resistance, swarming motility, and biofilm formation.