Three regulatory regions of the Aedes aegypti glutamine synthetase gene differentially regulate expression: identification of a crucial regulator in the first exon.
Three regulatory regions of the Aedes aegypti glutamine synthetase gene differentially regulate expression: identification of a crucial regulator in the first exon.
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埃及伊蚊谷氨酰胺合成酶基因的三个调节区域差异调节表达：第一个外显子中关键调节因子的鉴定。
DOI：
10.1046/j.1365-2583.2003.00442.x
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发表时间：
2003
影响因子：
2.6
通讯作者：
Christensen,BM
中科院分区：
文献类型：
--
作者：
Niu,LL;Kiley,LM;Dasgupta,R;Kohler,P;Christensen,BM
Aedes aegyptiglutamine synthetase (GS) is expressed constitutively at various developmental stages and its relative mRNA abundance increases in the midgut following blood feeding in support of the biosynthesis of chitin, a component of the peritrophic matrix. To understand the regulation of GS expression better, GS‐luciferase reporter fusion genes were constructed and analysed in transiently transfected C6/36 cells. These studies have identified three GS regions: GS‐A, ‐B and ‐C (C1, C2) that are required for efficient transcription. The crucial regulatory DNA sequence is located within 140 nucleotides of the GS‐C region in the first exon. GS‐B region between −209 and +4 contains a negative modulator that represses transcription of the GS‐C promoter, but the 5′‐GS‐A region, between −476 and −282, can negate the transcription inhibition of GS‐B and promote GS transcription of the GS‐C promoter. Electrophoretic mobility shift assays showed that nuclear proteins for GS‐A, GS‐B and GS‐C1 are present in the C6/36 cells, and therefore that GS‐A, GS‐B and GS‐C1 indeed possess regulatory function. By contrast, nuclear proteins isolated from both cultured cells and midgut tissues bound to GS‐C2, suggesting that GS‐C2 plays an important role in GS transcription and that GS‐C2 is regulated by several different and redundant transcription factors to achieve constitutive expression in a wide variety of tissues.
